RpS27A Antibody (OAAI00191)

Catalog No: OAAI00191

Size:100UG

IHC: Human Alzheimer brain stained with Anti- ubiquitin antibody, (Cat#OAAI00191 ) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Ubiquitin (Ub) is a highly conserved protein of approximately 8.5 kDa. It plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is covalently attached to C-term of ubiquitin, and the ubiquinated protein is then selectively degraded by a 26S complex, multicatalytic cytosolic and nuclear protease termed proteasome. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
  Positive Control: Human Brain from Alzheimer’sdisease
  Cellular Location: Nuclear, cytoplasmic, cellmembrane
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to C-term of ubiquitin protein.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: UBB
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: P M Hingamp, M L Leyland, J Webb, S Twigger, R J Mayer, and L K Dixon; Characterization of a ubiquitinated protein which is externally located in African swine fever virions. J Virol. 1995 March; 69(3): 1785–1793.
  

Target Info

• Gene Symbol: UBB
• Gene Full Name: ubiquitin B
• Alias Symbols: epididymis secretory protein Li 50;HEL-S-50;polyubiquitin B;polyubiquitin-B.
• NCBI Gene Id: 7314
• Protein Name: Ubiquitin-40S ribosomal protein S27a
• Description of Target: Ubiquitin (Ub) is a highly conserved protein of approximately 8.5 kDa. It plays a very important role in regulated non-ysosomal ATP dependent protein degradation. The protein to be degraded is covalently attached to C-term of ubiquitin, and the ubiquinated protein is then selectively degraded by a 26S complex, multicatalytic cytosolic and nuclear protease termed proteasome. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
• Uniprot ID: P15357
• Protein Accession #: NP_476778.1
• Nucleotide Accession #: NM_057430.4
• Molecular Weight: 8.5