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Datasheets/Manuals | Printable datasheet for RPL22 Antibody (OAEB00219) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | IHC |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | RPL22 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 84%; Horse: 84%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 92% |
Peptide Sequence | C-SKESYELRYFQINQ |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC: In paraffin embedded Human Brain (Cortex) shows cytoplasm staining in neuronal bodies. Recommended concentration, 3-6ug/ml |
Reference | * Anderson SJ, Lauritsen JP, Hartman MG, Foushee AM, Lefebvre JM, Shinton SA, Gerhardt B, Hardy RR, Oravecz T, Wiest DL.; Ablation of Ribosomal Protein L22 Selectively Impairs alphabeta T Development by Activation of a p53-Dependent Checkpoint.; Immunity. 2007 Jun;26(6):759-72. Epub 2007 Jun 7.; Pubmed 17555992 |
Gene Symbol | Rpl22|RPL22 |
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Gene Full Name | ribosomal protein L22 |
Alias Symbols | 2700038K18Rik;60S ribosomal protein L22;EAP;EBER-associated protein;Epstein-Barr virus small RNA-associated protein;Epstein-Barr-encoded RNA-associated protein;HBP15;HBP15/L22;heparin-binding protein 15;heparin-binding protein HBp15;L22;large ribosomal subunit protein eL22. |
NCBI Gene Id | 19934|6146|81768 |
Protein Name | 60S ribosomal protein L22 |
Uniprot ID | P35268 |
Protein Accession # | NP_000974.1 |
Molecular Weight | 14.8 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "RPL22 Antibody (OAEB00219)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "RPL22 Antibody (OAEB00219)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "RPL22 Antibody (OAEB00219)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "RPL22 Antibody (OAEB00219)"?
This target may also be called "2700038K18Rik;60S ribosomal protein L22;EAP;EBER-associated protein;Epstein-Barr virus small RNA-associated protein;Epstein-Barr-encoded RNA-associated protein;HBP15;HBP15/L22;heparin-binding protein 15;heparin-binding protein HBp15;L22;large ribosomal subunit protein eL22." in publications.
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What is the shipping cost for "RPL22 Antibody (OAEB00219)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "RPL22 Antibody (OAEB00219)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "RPL22 Antibody (OAEB00219)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "14.8".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "RPL22 Antibody (OAEB00219)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Rpl22|RPL22"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Rpl22|RPL22"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Rpl22|RPL22"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Rpl22|RPL22"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Rpl22|RPL22"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Rpl22|RPL22"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.