ROS1 ELISA Kit (Rat) (OKCD00198)

Catalog No: OKCD00198 (Formerly GWB-KBBAP9)

Size:96WELLS

Rat Ros1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	94-102%	85-93%	89-102%	78-99%
  EDTA plasma(n=5)	82-94%	97-105%	86-95%	85-92%
  heparin plasma(n=5)	82-98%	86-99%	96-104%	78-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	97-104	101
  EDTA plasma(n=5)	80-94	84
  heparin plasma(n=5)	84-96	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ros1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Ros1 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Ros1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 6.6 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1). No significant cross-reactivity or interference between C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Ros1
• Gene Full Name: ROS proto-oncogene 1 , receptor tyrosine kinase
• Alias Symbols: c-ros oncogene 1 , receptor tyrosine kinase;c-Ros receptor tyrosine kinase;c-ros-1;heart - derived c - ros - 1 proto - oncogene;proto-oncogene c-Ros;proto-oncogene c-Ros-1;proto-oncogene tyrosine-protein kinase ROS;proto-oncogene tyrosine-protein kinase ROS-like;receptor tyrosine kinase c-ros oncogene 1;Ros1 proto-oncogene;ROS1C;v-ros UR2 sarcoma virus oncogene homolog 1.
• NCBI Gene Id: 25346
• Protein Name: Proto-oncogene tyrosine-protein kinase ROS
• Description of Target: Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2 (By similarity).
• Uniprot ID: Q63132
• Protein Accession #: NP_037006
• Nucleotide Accession #: NM_012874.1
• Protein Size (# AA): 2338