RORC Antibody (OAEB00974)

Catalog No: OAEB00974 (Formerly GWB-EV1123)

Size:100UG

OAEB00974 (0.1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

(0.1ug/ml) staining of Human Colon (A), (0.03ug/ml) Liver (B) and (0.01ug/ml) Testes (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, cytoplasmic and plasma membrane staining.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: RORC
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Dog, Cow. This batch is not expected to cross-react with Mouse in Western Blot.
• Peptide Sequence: CHLEYSPERGKAE
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:16000
  WB: Approx 55 kDa band observed in Human Colon lysates (calculated MW of 55.8kDa according to NP_001001523.1). Recommended concentration: 0.1-0.3 ug/ml.
• Publications:

  Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth. Mucosal Immunol. 8, 1047-59 (2015). 25603827

• Description:
  View product citations for RORC Antibody (OAEB00974) on CiteAb

Target Info

• Gene Symbol: Rorc|RORC
• Gene Full Name: RAR related orphan receptor C|RAR-related orphan receptor gamma
• Alias Symbols: IMD42;NR1F3;nuclear receptor ROR-gamma;nuclear receptor RZR-gamma;nuclear receptor subfamily 1 group F member 3;RAR-related orphan nuclear receptor variant 2;RAR-related orphan receptor C;retinoic acid-binding receptor gamma;retinoid-related orphan receptor gamma;retinoid-related orphan receptor-gamma;ROR;RORG;RORgamma;RZRG;RZR-GAMMA;Th;Thor;thymus orphan receptor;TOR.
• NCBI Gene Id: 19885|6097
• Protein Name: Nuclear receptor ROR-gamma
• Uniprot ID: P51449
• Protein Accession #: NP_005051.2
• Molecular Weight: 58.2; 55.8