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Catalog No: OKCD02854
Size:96WELLS
Price: $675.00
SKU
OKCD02854
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Datasheets/ManualsPrintable datasheet for ROR1 ELISA Kit (Human) (OKCD02854)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ROR1 Microplate96 Wells (12 x 8 Well strips)
ROR1 Lyophilized Standard2
100X Biotinylated ROR1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1). No significant cross-reactivity or interference between Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolROR1
Gene Full Namereceptor tyrosine kinase like orphan receptor 1
Alias SymbolsdJ537F10.1;inactive tyrosine-protein kinase transmembrane receptor ROR1;neurotrophic tyrosine kinase, receptor-related 1;NTRKR1.
NCBI Gene Id4919
Protein NameInactive tyrosine-protein kinase transmembrane receptor ROR1
Description of TargetHas very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo (PubMed:25029443). Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling (PubMed:25029443, PubMed:27162350). In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells (PubMed:27162350).
Uniprot IDQ01973
Protein Accession #NP_001077061.1
Nucleotide Accession #NM_001083592.1
Protein Size (# AA)937
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