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Catalog No: OKEH01371
Size:96T
Price: $525.00
SKU
OKEH01371
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01371
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ROMO1 ELISA Kit (Human) (OKEH01371) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ROMO1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ROMO1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ROMO1 captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
ROMO1 Microplate96 Wells (12 x 8 Well strips)
ROMO1 Lyophilized Standard2
100X Biotinylated ROMO1 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.13 ng/mL
SpecificityNatural and recombinant Human Reactive oxygen species modulator 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolROMO1
Gene Full Namereactive oxygen species modulator 1
Alias SymbolsbA353C18.2;C20orf52;epididymis tissue protein Li 175;glyrichin;mitochondrial targeting GxxxG motif protein;mitochondrial targeting GXXXG protein;MTGM;MTGMP;PCM19;protein MGR2 homolog;reactive oxygen species modulator 1;ROS modulator 1.
NCBI Gene Id140823
Protein NameP60602
Description of TargetReactive oxygen species modulator 1
Uniprot IDhttps://www.uniprot.org/uniprot/P60602
Protein Accession #NP_542786.1
Nucleotide Accession #NM_080748.2
Protein Size (# AA)79
Molecular Weight8 kDa
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