RNASE1 ELISA Kit (Human) (OKCD01226)

Catalog No: OKCD01226 (Formerly GWB-KBBPL1)

Size:96WELLS

Human RNASE1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	85-94%	94-105%	88-102%	93-105%
  EDTA plasma(n=5)	93-101%	93-105%	91-101%	97-105%
  heparin plasma(n=5)	78-97%	89-103%	86-94%	86-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A (RNase A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A (RNase A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A (RNase A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ribonuclease A (RNase A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	88-97	93
  EDTA plasma(n=5)	84-95	88
  heparin plasma(n=5)	80-96	81

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonuclease A (RNase A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonuclease A (RNase A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-RNASE1 Microplate: 96 Wells (12 x 8 Well Strips)
  • RNASE1 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated RNASE1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 37 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ribonuclease A (RNASE1). No significant cross-reactivity or interference between Ribonuclease A (RNASE1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: RNASE1
• Gene Full Name: ribonuclease A family member 1, pancreatic
• Alias Symbols: HP-RNase;RAC1;RIB1;RIB-1;ribonuclease 1;Ribonuclease A;ribonuclease A C1;ribonuclease pancreatic;ribonuclease, RNase A family, 1 (pancreatic);RNase 1;RNase A;RNase upI-1;RNS1.
• NCBI Gene Id: 6035
• Protein Name: Ribonuclease pancreatic
• Description of Target: Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.
• Uniprot ID: P07998
• Protein Accession #: NP_002924
• Nucleotide Accession #: NM_002933.4
• Protein Size (# AA): 156