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Catalog No: OKEH00878
Size:96T
Price: $625.00
SKU
OKEH00878
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology RLN ELISA Kit (Pig) (OKEH00878) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RLN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RLN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RLN captured in well.
ELISA Kit Range62.5-4000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
RLN Microplate96 Wells (12 x 8 Well strips)
RLN Lyophilized Standard2
100X Biotinylated RLN Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity31.25 pg/mL
SpecificityNatural and recombinant Pig Prorelaxin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRLN2
Gene Full Namerelaxin 2
Alias Symbolspreprorelaxin;prorelaxin;RLN.
NCBI Gene Id396891
Protein NameP01348
Description of TargetProrelaxin
Uniprot IDhttps://www.uniprot.org/uniprot/P01348
Protein Accession #NP_999037.1
Nucleotide Accession #NM_213872.1
Protein Size (# AA)182
Molecular Weight21 kDa
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