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Catalog No: OKDD00507 (Formerly GWB-KBBVJ6)
Size:96 Wells
Price: $800.00
SKU
OKDD00507
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Datasheets/ManualsPrintable datasheet for RIPK1 ELISA Kit (Human) (OKDD00507)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology RIPK1 ELISA Kit (Human) (OKDD00507) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for RIPK1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RIPK1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RIPK1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RIPK1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RIPK1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-RIPK1 Microplate96 Wells (12 x 8 Well Strips)
RIPK1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X RIPK1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, cell lysates
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of RIPK1. No significant cross-reactivity or interference between RIPK1 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolRIPK1
Gene Full Namereceptor interacting serine/threonine kinase 1
Alias SymbolsAIEFL;cell death protein RIP;IMD57;receptor (TNFRSF)-interacting serine-threonine kinase 1;receptor-interacting protein 1;receptor-interacting protein kinase 1;receptor-interacting serine/threonine-protein kinase 1;RIP;RIP1;RIP-1;serine/threonine-protein kinase RIP.
NCBI Gene Id8737
Protein NameReceptor-interacting serine/threonine-protein kinase 1
Description of TargetSerine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.
Uniprot IDQ13546
Protein Accession #NP_001303990.1
Nucleotide Accession #NM_001317061.1
Protein Size (# AA)671
Molecular Weight76 kDa
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