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Catalog No: OKEH02090
Size:96T
Price: $525.00
SKU
OKEH02090
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology RGS1 ELISA Kit (Human) (OKEH02090) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RGS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RGS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RGS1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
RGS1 Microplate96 Wells (12 x 8 Well strips)
RGS1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated RGS1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Regulator of G-protein signaling 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRGS1
Gene Full Nameregulator of G protein signaling 1
Alias Symbols1R20;B-cell activation protein BL34;BL34;early response protein 1R20;epididymis secretory protein Li 87;HEL-S-87;IER1;immediate-early response 1, B-cell specific;IR20;regulator of G-protein signaling 1;regulator of G-protein signalling 1.
NCBI Gene Id5996
Protein NameQ08116
Description of TargetRegulator of G-protein signaling 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q08116
Protein Accession #NP_002913.3
Nucleotide Accession #NM_002922.3
Protein Size (# AA)209
Molecular Weight24 kDa
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