RGCC ELISA Kit (Human) (OKCD01083)

Catalog No: OKCD01083 (Formerly GWB-KBBJD1)

Size:96WELLS

Human RGCC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Response Gene To Complement 32 (RGC32). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Response Gene To Complement 32 (RGC32). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Response Gene To Complement 32 (RGC32), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Response Gene To Complement 32 (RGC32) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Response Gene To Complement 32 (RGC32) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Response Gene To Complement 32 (RGC32) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-RGCC Microplate: 96 Wells (12 x 8 Well Strips)
  • RGCC Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated RGCC Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.128 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Response Gene To Complement 32 (RGC32). No significant cross-reactivity or interference between Response Gene To Complement 32 (RGC32) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: RGCC
• Gene Full Name: regulator of cell cycle
• Alias Symbols: bA157L14.2;C13orf15;regulator of cell cycle RGCC;response gene to complement 32 protein;RGC32;RGC-32.
• NCBI Gene Id: 28984
• Protein Name: Regulator of cell cycle RGCC
• Description of Target: Modulates the activity of cell cycle-specific kinases. Enhances CDK1 activity. May contribute to the regulation of the cell cycle. May inhibit growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. Fibrogenic factor contributing to the pathogenesis of renal fibrosis through fibroblast activation.
• Uniprot ID: Q9H4X1
• Protein Accession #: NP_054778
• Nucleotide Accession #: NM_014059.2
• Protein Size (# AA): 137