RFC Polyclonal Antibody (OABB00668)

Catalog No: OABB00668 (Formerly GWB-BBP591)

Size:100UG

Lane 1：Hela Cell Lysate
Lane 2：SMMC Cell Lysate
Lane 3：Jurkat Cell Lysate
Lane 4：CEM Cell Lysate
Lane 5：293T Cell Lysate

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: RFC(Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
  Background: RFC (Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40 (SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Reference: 1. Bermudez, V. P., Maniwa, Y., Tappin, I., Ozato, K., Yokomori, K., Hurwitz, J. The alternative
  Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads
  proliferating cell nuclear antigen onto DNA. Proc. Nat. Acad. Sci. 100: 10237-10242, 2003.
  2. Peoples, R., Perez-Jurado, L., Wang, Y.-K., Kaplan, P., Francke, U. The gene for replication factor C
  subunit 2 (RFC2) is within the 7q11.23 Williams syndrome deletion. (Letter)Am. J. Hum. Genet. 58:
  1370-1373, 1996
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P, FCM in Human;Mouse;Rat.

Target Info

• Gene Symbol: RFC1
• Gene Full Name: replication factor C subunit 1
• Alias Symbols: A1;A1 140 kDa subunit;activator 1 140 kDa subunit;activator 1 large subunit;activator 1 subunit 1;CANVAS;DNA-binding protein PO-GA;MHC binding factor, beta;MHCBFB;PO-GA;RECC1;replication factor C (activator 1) 1, 145kDa;replication factor C 140 kDa subunit;replication factor C large subunit;replication factor C subunit 1;replication factor C1;RFC;RF-C 140 kDa subunit;RFC140.
• NCBI Gene Id: 5981
• Protein Name: Replication factor C subunit 1
• Description of Target: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
• Uniprot ID: P35251
• Molecular Weight: 128255 MW