Catalog No: OKCD01183 (Formerly GWB-KBBAT2)
Size:96WELLS
Price: $750.00
SKU
OKCD01183
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Datasheets/ManualsPrintable datasheet for RETNLB ELISA Kit (Mouse) (OKCD01183)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-91%82-96%85-94%93-101%
EDTA plasma(n=5)95-103%82-96%79-94%97-105%
heparin plasma(n=5)92-99%78-104%97-105%87-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin Like Beta (RETNLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin Like Beta (RETNLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin Like Beta (RETNLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Resistin Like Beta (RETNLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range6.25-400pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)95-10498
EDTA plasma(n=5)85-9994
heparin plasma(n=5)87-10496
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin Like Beta (RETNLb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin Like Beta (RETNLb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Retnlb Microplate96 Wells (12 x 8 Well Strips)
Retnlb Lyophilized Standard2 x 400 pg
100X Biotinylated Retnlb Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 2.54 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Resistin Like Beta (RETNLb). No significant cross-reactivity or interference between Resistin Like Beta (RETNLb) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolRetnlb
Gene Full Nameresistin like beta
Alias Symbols9030012B21Rik;cysteine-rich secreted protein A12-beta;cysteine-rich secreted protein FIZZ2;Fizz;Fizz2;found in inflammatory zone 2;Rel;Relmb;RELMbeta;resistin-like beta;resistin-like molecule beta;Xcp;Xcp3.
NCBI Gene Id57263
Protein NameResistin-like beta
Description of TargetProbable hormone.
Uniprot IDQ99P86
Protein Accession #NP_076370
Nucleotide Accession #NM_023881.4
Protein Size (# AA)105
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