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RUVBL1 antibody - N-terminal region (ARP32349_P050)

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Description of Target:
RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is the component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
Gene Symbol:
RUVBL1
Official Gene Full Name:
RuvB-like 1 (E. coli)
NCBI Gene Id:
8607
Alias Symbols:
ECP54; NMP238; Pontin52; RVB1; TIH1; TIP49; TIP49A; INO80H; PONTIN
Tissue Tool:
Find tissues and cell lines supported to express RUVBL1.
Protein Accession #:
NP_003698
Nucleotide Accession#:
NM_003707
Swissprot Id:
Q9Y265
Host:
Rabbit
Clonality:
Polyclonal
Protein Name:
RuvB-like 1
Protein Size (# AA):
456
Molecular Weight:
50kDa
Application:
WB
Partner Proteins:
ASCL1, BMP2, EP300, MEF2A, NEUROG2, TCF4, UBQLN1, EP300, MEF2A, TCF4
Immunogen:
The immunogen for anti-RUVBL1 antibody: synthetic peptide directed towards the N terminal of human RUVBL1
Product Format:
Lyophilized powder
Purification:
Affinity Purified
Complete computational species homology data:
RUVBL1 antibody - N-terminal region (ARP32349_P050)
Predicted Homology Based on Immunogen Sequence:
Dog: 100%; Pig: 100%; Rat: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Bovine: 100%; Rabbit: 100%; Zebrafish: 100%; Guinea pig: 100%; Goat: 77%
Species Reactivity:
Bovine, Human, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse, Zebrafish, Goat
Datasheets / Downloads:
Printable datasheet for
anti-RUVBL1 antibody
- ARP32349_P050
Peptide Sequence:
Synthetic peptide located within the following region: KIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGV
Blocking Peptide:
For anti-RUVBL1 antibody is Catalog # AAP32349 (Previous Catalog # AAPP03338)
Target Reference:
Jha,S., (2008) Mol. Cell. Biol. 28 (8), 2690-2700
Reconstitution and Storage:
Add 50 ul of distilled water. Final anti-RUVBL1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20C. Avoid repeat freeze-thaw cycles.

Computational species homology for RUVBL1 antibody (ARP32349)

Product page for RUVBL1 antibody (ARP32349)

The information below lists the predicted species and target name associated to the peptide antigen. Please note, all available target reference numbers to the antigen sequence are presented, including unreviewed and protein isoforms.
To search for antibodies by species, please visit Aviva’s Species Reactivity Page. To search Aviva’s catalog of antibodies by sequence, please visit Aviva’s Antibody Blast Tool.

Predicted Species & Target Target Reference Predicted Homology
African clawed frog RUVB1 antibody; Xenopus laevis RUVB1 antibody Q9DE26 100%
African clawed frog ruvbl1 antibody; Xenopus laevis ruvbl1 antibody Q6GR29 100%
African elephant RUVBL1 antibody; Loxodonta africana RUVBL1 antibody G3TIT2 100%
Albugo laibachii Nc14 AlNc14C284G10152 antibody F0WV07 78%
Atlantic salmon ruvb1 antibody; Salmo salar ruvb1 antibody B5X3S2 92%
Bovine RUVBL1 antibody; Bos taurus RUVBL1 antibody A7MBG8 100%
Chicken RUVBL1 antibody; Gallus gallus RUVBL1 antibody Q5ZIC4 92%
Chicken RUVBL1 antibody; Gallus gallus RUVBL1 antibody F1N8Z4 92%
Chimpanzee RUVBL1 antibody; Pan troglodytes RUVBL1 antibody G2HJD7 100%
Chinese hamster LOC100761458 antibody; Cricetulus griseus LOC100761458 antibody G3HIC5 100%
Common turkey LOC100550454 antibody; Meleagris gallopavo LOC100550454 antibody G1N5W0 92%
Dog RUVBL1 antibody; Canis familiaris RUVBL1 antibody E2RQC9 100%
Fruit fly DanaGF17460 antibody; Drosophila ananassae DanaGF17460 antibody B3LW03 84%
Fruit fly DereGG17489 antibody; Drosophila erecta DereGG17489 antibody B3P1P6 84%
Fruit fly DgriGH19121 antibody; Drosophila grimshawi DgriGH19121 antibody B4JIM2 84%
Fruit fly DmojGI23576 antibody; Drosophila mojavensis DmojGI23576 antibody B4K538 84%
Fruit fly DperGL23687 antibody; Drosophila persimilis DperGL23687 antibody B4G6B2 84%
Fruit fly DsecGM23872 antibody; Drosophila sechellia DsecGM23872 antibody B4HJ74 84%
Fruit fly DsimGD18681 antibody; Drosophila simulans DsimGD18681 antibody B4QV95 84%
Fruit fly DvirGJ23553 antibody; Drosophila virilis DvirGJ23553 antibody B4LWC5 84%
Fruit fly DwilGK12828 antibody; Drosophila willistoni DwilGK12828 antibody B4NJQ0 84%
Fruit fly DyakGE26023 antibody; Drosophila yakuba DyakGE26023 antibody B4PKN3 84%
Fruit fly RUVB1 antibody; Drosophila melanogaster RUVB1 antibody Q9VH07 84%
Fruit fly RUVB1 antibody; Drosophila pseudoobscura pseudoobscura RUVB1 antibody Q29AK9 84%
Giant panda RUVBL1 antibody; Ailuropoda melanoleuca RUVBL1 antibody D2H7C0 100%
Gray short-tailed opossum RUVBL1 antibody; Monodelphis domestica RUVBL1 antibody F7CL07 100%
Green alga ponA antibody; Volvox carteri ponA antibody D8UDK7 84%
Guinea pig LOC100720747 antibody; Cavia porcellus LOC100720747 antibody H0VEU3 100%
Horse RUVBL1 antibody; Equus caballus RUVBL1 antibody F7DVX3 100%
Human RUVB1 antibody; Homo sapiens RUVB1 antibody Q9Y265 100%
Human RUVB1 antibody; Homo sapiens RUVB1 antibody Q9Y265-2 100%
Human RUVBL1 antibody; Homo sapiens RUVBL1 antibody B5BUB1 100%
Little brown bat RUVBL1 antibody; Myotis lucifugus RUVBL1 antibody G1P201 100%
Lowland gorilla RUVBL1 antibody; Gorilla gorilla gorilla RUVBL1 antibody G3R4Q3 100%
Moss RUVBL1501 antibody; Physcomitrella patens subsp. patens RUVBL1501 antibody A9RE67 78%
Mouse RUVB1 antibody; Mus musculus RUVB1 antibody P60122 100%
Mouse Ruvbl1 antibody; Mus musculus Ruvbl1 antibody Q3UJN2 100%
Mouse Ruvbl1 antibody; Mus musculus Ruvbl1 antibody Q3U1C2 100%
Northern white-cheeked gibbon RUVBL1 antibody; Nomascus leucogenys RUVBL1 antibody G1RVN0 100%
Rabbit RUVBL1 antibody; Oryctolagus cuniculus RUVBL1 antibody G1TBS2 100%
Rat RUVB1 antibody; Rattus norvegicus RUVB1 antibody P60123 100%
Red flour beetle pont antibody; Tribolium castaneum pont antibody D6X0K0 91%
Rhesus macaque RUVBL1 antibody; Macaca mulatta RUVBL1 antibody F6ZZE0 100%
Rice OJ1014_E09.2 antibody; Oryza sativa subsp. japonica OJ1014_E09.2 antibody Q6ZLD7 84%
salmon louse RUVB1 antibody; Lepeophtheirus salmonis RUVB1 antibody D3PGS6 83%
Small-eared galago RUVBL1 antibody; Otolemur garnettii RUVBL1 antibody H0XAG7 100%
Three-spined stickleback RUVBL1 antibody; Gasterosteus aculeatus RUVBL1 antibody G3N6U1 100%
Transparent sea squirt LOC100183367 antibody; Ciona intestinalis LOC100183367 antibody F6R8X1 100%
Volvox carteri f. nagariensis ponA antibody A1YQY4 84%
Western clawed frog ruvbl1 antibody; Xenopus tropicalis ruvbl1 antibody Q28FH8 100%
Western clawed frog ruvbl1 antibody; Xenopus tropicalis ruvbl1 antibody F6Q5T7 100%
Western clawed frog ruvbl1 antibody; Xenopus tropicalis ruvbl1 antibody F6Q5K9 100%
White-tufted-ear marmoset RUVBL1 antibody; Callithrix jacchus RUVBL1 antibody F7GWT4 100%
White-tufted-ear marmoset RUVBL1 antibody; Callithrix jacchus RUVBL1 antibody F7GWQ2 100%
White-tufted-ear marmoset RUVBL1 antibody; Callithrix jacchus RUVBL1 antibody F6TUT5 100%
Yellowfever mosquito RUVB1 antibody; Aedes aegypti RUVB1 antibody Q0IFL2 92%
Zebra finch LOC100225975 antibody; Taeniopygia guttata LOC100225975 antibody H0ZC84 92%
Zebrafish RUVB1 antibody; Danio rerio RUVB1 antibody Q8AWW7 100%
Zebrafish ruvbl1 antibody; Danio rerio ruvbl1 antibody Q6TGU6 100%
Zebrafish ruvbl1 antibody; Danio rerio ruvbl1 antibody Q6IQT8 100%

Product Protocols: RUVBL1 antibody tested with Human Fetal Heart Tissue (ARP32349_P050)

Aviva Systems Biology is the original manufacturer of this RUVBL1 antibody (ARP32349_P050)

Click here to view the RUVBL1 antibody Western Blot Protocol

Product Datasheet Link: RUVBL1 antibody (ARP32349_P050)

WB Suggested Anti-RUVBL1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Fetal Heart

Western Blot image:


Description of Target: RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is the component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s RUVBL1 antibody (ARP32349_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Review: RUVBL1 antibody - N-terminal region (ARP32349_P050) in K562 lysate using Western Blot

Product Page for RUVBL1 antibody - N-terminal region (ARP32349_P050)

Researcher: Sustackova Gabriela
Application: Western Blotting
Species+tissue/cell type: Lane 1: 30ug K562 lysate
Primary antibody dilution:1:200
Secondary antibody: Anti-rabbit-HRP
Secondary antibody dilution: 1:1000

Questionnaire:
How do Aviva’s reagents play a role in your experimental goals? I wanted to find antibody which would work for Drosophila cells
Have you used another antibody which has worked in your application? Yes
Do you believe the information about the reagent on Aviva’s website is correct? Yes
How did you store the antibody after re-suspension? In aliquots at -20°C
Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): 1) Drosophila, 2) Drosophila S2 cells, 3) 30 μg
How many different experimental trials were conducted using the antibody sample? 7
How was this sample prepared? Cells were lysed by sample buffer (50 mM Tris, 10% glycerol, 1% SDS), then sonicated and boiled with 2-mercaptoethanol and bromophenol blue, storage at -20°C
Primary antibody dilution and incubation time: 1:200 in 5% milk, incubated either overnight at 4°C or 1h at room temperature
Secondary antibody used and dilution and incubation time: Goat anti-rabbit conjugated with HRP, incubated for 1h at room temperature
What controls were used in your experiment (positive/negative)? Positive control - K562 cells/ negative control - knockdown of gene in Drosophila cells using dsRNA
Please include your detailed WB Procedure/Protocol here: Samples ran in 12% SDS-polyacrylamide gel at 100-130V. Proteins were transferred from gel to membrane in 1h 30min at 300mA. Blocking - 1h at room temperature in 5% milk. Incubation with RUVBL1 antibody - either overnight at 4°C or 1h at room temperature. Dilution 1:200 in 5% milk. Washing - 3x 10 minutes in PBST buffer (0.05%). Incubation with secondary antibody - 1h at room temperature. Dilution of goat-anti-rabbit - HRP antibody 1:1000 in 5% milk. Washing - 3x 10 minutes in PBST (0.05%). Developed by Clarity Western ECL substrate (Biorad) and exposed to film.
comment to the image:
line 1 - K562 positive control
line 2 - S2 cells control
line 3 - S2 cells with silenced RUVBL1
RUVBL1 should show band around 50 kDa, however in S2 cells there wasn't any band about this size even with high concentration of antibody and loading of 30 μg of protein sample. In K562 leukemic cells there was a band above 55 kDa (MW marker PageRuler Plus Prestained Protein Ladder, Thermo Scientific).
 
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