RUVBL1 antibody - N-terminal region (ARP32349_P050)

Catalog#: ARP32349_P050

• Size: 50ug

Human Heart

• Description of Target: RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is the component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
• Gene Symbol: RUVBL1
• Official Gene Full Name: RuvB-like 1 (E. coli)
• Alias Symbols: ECP54; NMP238; Pontin52; RVB1; TIH1; TIP49; TIP49A; INO80H; PONTIN
• Protein Accession#: NP_003698
• Nucleotide Accession#: NM_003707
• Swissprot Id: Q9Y265
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 456
• Molecular Weight: 50kDa
• Application: WB
• Partner Proteins: ASCL1, BMP2, EP300, MEF2A, NEUROG2, TCF4, UBQLN1, EP300, MEF2A, TCF4
• Immunogen: The immunogen for anti-RUVBL1 antibody: synthetic peptide directed towards the N terminal of human RUVBL1
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: African clawed frog: 100%; Bovine: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Zebrafish: 100%; Chicken: 92%
• Peptide Sequence: Synthetic peptide located within the following region: KIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGV
• Blocking Peptide: For anti-RUVBL1 antibody is Catalog # AAP32349 (Previous Catalog # AAPP03338)
• Key Reference: Jha,S., (2008) Mol. Cell. Biol. 28 (8), 2690-2700
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-RUVBL1 antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: RUVBL1 antibody tested with Human Fetal Heart Tissue (ARP32349_P050)

Aviva Systems Biology is the original manufacturer of this RUVBL1 antibody (ARP32349_P050)

Click here to view the RUVBL1 antibody Western Blot Protocol

Product Datasheet Link: RUVBL1 antibody (ARP32349_P050)

WB Suggested Anti-RUVBL1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Fetal Heart

Western Blot image:


Description of Target: RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is the component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s RUVBL1 antibody (ARP32349_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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Product Review: RUVBL1 antibody - N-terminal region (ARP32349_P050) in K562 lysate using Western Blot

Product Page for RUVBL1 antibody - N-terminal region (ARP32349_P050)

Researcher: Sustackova Gabriela
Application: Western Blotting
Species+tissue/cell type: Lane 1: 30ug K562 lysate
Primary antibody dilution:1:200
Secondary antibody: Anti-rabbit-HRP
Secondary antibody dilution: 1:1000

Questionnaire:
How do Aviva’s reagents play a role in your experimental goals?	I wanted to find antibody which would work for Drosophila cells
Have you used another antibody which has worked in your application?	Yes
Do you believe the information about the reagent on Aviva’s website is correct?	Yes
How did you store the antibody after re-suspension?	In aliquots at -20°C
Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel):	1) Drosophila, 2) Drosophila S2 cells, 3) 30 μg
How many different experimental trials were conducted using the antibody sample?	7
How was this sample prepared?	Cells were lysed by sample buffer (50 mM Tris, 10% glycerol, 1% SDS), then sonicated and boiled with 2-mercaptoethanol and bromophenol blue, storage at -20°C
Primary antibody dilution and incubation time:	1:200 in 5% milk, incubated either overnight at 4°C or 1h at room temperature
Secondary antibody used and dilution and incubation time:	Goat anti-rabbit conjugated with HRP, incubated for 1h at room temperature
What controls were used in your experiment (positive/negative)?	Positive control - K562 cells/ negative control - knockdown of gene in Drosophila cells using dsRNA
Please include your detailed WB Procedure/Protocol here:	Samples ran in 12% SDS-polyacrylamide gel at 100-130V. Proteins were transferred from gel to membrane in 1h 30min at 300mA. Blocking - 1h at room temperature in 5% milk. Incubation with RUVBL1 antibody - either overnight at 4°C or 1h at room temperature. Dilution 1:200 in 5% milk. Washing - 3x 10 minutes in PBST buffer (0.05%). Incubation with secondary antibody - 1h at room temperature. Dilution of goat-anti-rabbit - HRP antibody 1:1000 in 5% milk. Washing - 3x 10 minutes in PBST (0.05%). Developed by Clarity Western ECL substrate (Biorad) and exposed to film. comment to the image: line 1 - K562 positive control line 2 - S2 cells control line 3 - S2 cells with silenced RUVBL1 RUVBL1 should show band around 50 kDa, however in S2 cells there wasn't any band about this size even with high concentration of antibody and loading of 30 μg of protein sample. In K562 leukemic cells there was a band above 55 kDa (MW marker PageRuler Plus Prestained Protein Ladder, Thermo Scientific).