TPM1 antibody - N-terminal region (ARP41392_T100)

Catalog#: ARP41392_T100

• Size: 100ug

Human HepG2

Human Muscle

• Description of Target: TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction.This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.
• Gene Symbol: TPM1
• Official Gene Full Name: Tropomyosin 1 (alpha)
• Alias Symbols: CMH3; TMSA; TPM1-alpha; CMD1Y; C15orf13; HTM-alpha
• Protein Accession#: NP_000357
• Nucleotide Accession#: NM_000366
• Swissprot Id: P09493
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 284
• Molecular Weight: 31kDa
• Application: WB, IHC
• Partner Proteins: C1QA, C1QB, FCGR1A, FGA, PLG, THBS1, FGA
• Immunogen: The immunogen for anti-TPM1 antibody: synthetic peptide directed towards the N terminal of human TPM1
• Product Format: Lyophilized powder
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: African clawed frog: 100%; Chicken: 100%; Dog: 100%; Guinea pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%; Bovine: 92%; Pig: 92%; Sheep: 92%
• Peptide Sequence: Synthetic peptide located within the following region: DRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQE
• Blocking Peptide: For anti-TPM1 antibody is Catalog # AAP41392 (Previous Catalog # AAPP24130)
• Key Reference: Lin,J.C. (2005) Mol. Cell. Biol. 25 (22), 10111-10121
• Reconstitution and Storage: Add 100 μl of distilled water. Final anti-TPM1 antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: TPM1 antibody tested with Human Hepg2 Cells (ARP41392_T100)

Aviva Systems Biology is the original manufacturer of this TPM1 antibody (ARP41392_T100)

Click here to view the TPM1 antibody Western Blot Protocol

Product Datasheet Link: TPM1 antibody (ARP41392_T100)

WB Suggested Anti-TPM1 Antibody Titration: 1.25ug/ml
Positive Control: HepG2

Western Blot image:


Description of Target: TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction.This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.

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Aviva Systems Biology’s TPM1 antibody (ARP41392_T100) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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Product Protocols: TPM1 antibody tested by IHC with human muscle (ARP41392)

Aviva Systems Biology is the original manufacturer of this TPM1 antibody.

Click here to view the TPM1 antibody Immunohistochemistry (IHC) protocol

Product Datasheet Link: TPM1 antibody (ARP41392)

IHC Information:

Rabbit Anti-TPM1 Antibody
Catalog Number: ARP41392
Paraffin Embedded Tissue: Human Muscle
Cellular Data: Skeletal muscle cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

IHC Image: