- Description of Target:
- The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene.
- Gene Symbol:
- Official Gene Full Name:
- Solute carrier family 12 (sodium/potassium/chloride transporters), member 1
- NCBI Gene Id:
- Alias Symbols:
- BSC1, MGC48843, NKCC2
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express SLC12A1.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express SLC12A1.
- Swissprot Id:
- Protein Accession #:
- Nucleotide Accession #:
- Protein Name:
- SLC12A1 protein EMBL AAH40138.1
- Protein Size (# AA):
- Molecular Weight:
- IHC, WB
- Protein Interactions:
- STK39; OXSR1;
- The immunogen is a synthetic peptide directed towards the N terminal region of human SLC12A1
- Product Format:
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
- Affinity Purified
- Complete computational species homology data:
- Anti-SLC12A1 (ARP41388_P050)
- Predicted Homology Based on Immunogen Sequence:
- Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
- Species Reactivity:
- Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
- Datasheets / Downloads:
- Printable datasheet for anti-SLC12A1 (ARP41388_P050) antibody
- Peptide Sequence:
- Synthetic peptide located within the following region: NEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANI
- Blocking Peptide:
- For anti-SLC12A1 (ARP41388_P050) antibody is Catalog # AAP41388 (Previous Catalog # AAPP24126)
- Reconstitution and Storage:
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Kelsen, S. et al. Heme oxygenase attenuates angiotensin II-mediated superoxide production in cultured mouse thick ascending loop of Henle cells. Am. J. Physiol. Renal Physiol. 295, F1158-65 (2008). WB, Mouse, Rat, Human, Dog, Pig, Horse, Rabbit, Bovine, Guinea pig 18701634
Stec, D. E. et al. Expression of heme oxygenase-1 in thick ascending loop of henle attenuates angiotensin II-dependent hypertension. J. Am. Soc. Nephrol. 23, 834-41 (2012). WB, Mouse, Rat, Human, Dog, Pig, Horse, Rabbit, Bovine, Guinea pig 22323644
Aviva Systems Biology is the original manufacturer of this SLC12A1 antibody (ARP41388_P050)
Click here to view the SLC12A1 antibody Western Blot Protocol
Product Datasheet Link: SLC12A1 antibody (ARP41388_P050)
WB Suggested Anti-SLC12A1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat
Western Blot image:
Description of Target: The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene.
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Aviva Systems Biology’s SLC12A1 antibody (ARP41388_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at firstname.lastname@example.org.
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Aviva Systems Biology is the original manufacturer of this SLC12A1 antibody.
Click here to view the SLC12A1 antibody Immunohistochemistry (IHC) protocol
Product Datasheet Link: SLC12A1 antibody (ARP41388)
Rabbit Anti-SLC12A1 Antibody
Catalog Number: ARP41388
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml