EGR1 Antibody - N-terminal region (ARP36841_T100)

Catalog No: ARP36841_T100

WB Suggested Anti-EGR1 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:12500
Positive Control: NIH/3T3 cell lysate

Sample Type:
Frog brain
Primary Antibody Dilution:
1:500
Secondary Antibody:
Biotinylated goat anti-rabbit
Secondary Antibody Dilution:
1:200
Color/Signal Descriptions:
Black: EGF1
Gene Name:
EGR1 a
Submitted by:
Eva Fischer, Colorado State University

Sample Type:
Frog brain
Primary Antibody Dilution:
1:500
Secondary Antibody:
Biotinylated goat anti-rabbit
Secondary Antibody Dilution:
1:200
Color/Signal Descriptions:
Black: EGF1
Gene Name:
EGR1 a
Submitted by:
Eva Fischer, Colorado State University

Product Info

• Tested Species Reactivity: Mouse, Frog
• Predicted Species Reactivity: Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Sheep
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of mouse EGR1
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 75%; Horse: 85%; Mouse: 100%; Pig: 85%; Rabbit: 77%; Rat: 100%; Sheep: 92%
• Peptide Sequence: Synthetic peptide located within the following region: GTPEGSGGNSSSSTSSGGGGGGGSNSGSSAFNPQGEPSEQPYEHLTTESF
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-EGR1 (ARP36841_T100) antibody is Catalog # AAP36841 (Previous Catalog # AAPP09914)
• Reference: Schnell,F.J., et al., (2006) J. Immunol. 176 (8), 4740-4747
• Publications:

  Anti-Egr-1 ARP36841_T100 has recently been referenced in the following publications:

  Revest, J.-M. et al. The enhancement of stress-related memory by glucocorticoids depends on synapsin-Ia/Ib. Mol. Psychiatry 15, 1125, 1140–51 (2010). WB, Mouse, Rat 20368707

Target Info

• Gene Symbol: EGR1
• Gene Full Name: Early growth response 1
• Alias Symbols: NG, TI, NGF, Zen, egr, Egr-, Krox, NGF1, TIS8, Zenk, Zfp-, ETR10, Egr-1, Krox-, NGFIA, Zfp-6, Zif26, ETR103, Krox-1, Krox24, NGF1-A, NGFI-A, Zif268, Krox-24, A530045N19Rik
• NCBI Gene Id: 13653
• Protein Name: Early growth response protein 1
• Description of Target: Early Growth Response Protein 1 (EGR1, Krox-24 protein, nerve growth factor-induced protein A, Transcription factor ETR103, Zinc finger protein 225) belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.
• Uniprot ID: P08046
• Protein Accession #: NP_031939
• Nucleotide Accession #: NM_007913
• Protein Size (# AA): 533
• Molecular Weight: 59kDa
• Protein Interactions: Sp1; Pin1; Sf1; Pitx1; Nab2; Egr1; G3bp1; Srf; Smarca4; Elk1; Hist4h4; Nelfe; Tbp; Gtf2b; Cdk8;