PAX4 Antibody - middle region (OAAB03746)

Catalog No: OAAB03746

Size:400UL

Western blot analysis of PAX4 antibody (Center) (RB20216) in CEM cell lysates (35ug/lane). PAX4 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with PAX4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of WiDr cells using PAX4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: Flow cytometry, Immunohistochemistry-Paraffin, Western blot
  Cellular Location: Nucleus.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PAX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human PAX4.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Application Info: WB: 1:2000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of widr cells using PAX4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: PAX4
• Alias Symbols: KPD, MODY9
• NCBI Gene Id: 5078
• Protein Name: NP_006184.2, O43316, P32115
• Description of Target: Paired box protein Pax-4, paired box protein Pax-4 [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/?query=O43316+OR+P32115
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_006184.2
• Molecular Weight: 37833