GARS antibody - middle region (ARP58758_P050)

Catalog#: ARP58758_P050

• Size: 50ug

Human MCF-7

• Description of Target: GARS catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Gene Symbol: GARS
• Official Gene Full Name: Glycyl-tRNA synthetase
• Alias Symbols: CMT2D; DSMAV; GlyRS; HMN5; SMAD1
• Protein Accession#: NP_002038
• Nucleotide Accession#: NM_002047
• Swissprot Id: P41250
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 739
• Molecular Weight: 83kDa
• Application: WB
• Partner Proteins: EPS8, FGFR1, PDGFRB, PIK3R1, SRC, src, CRK, EPS8, GRAP2, GRB2, IL2RB, IL2RG, JAK1, JAK3, KDR, LAT, LCP2, PDGFRA, PIK3R1, PLCG1, SLC39A1, SRC, VAV1, ZAP70, CRK, EPS8, FGFR1, GRB2, IL2RB, IL2RG, JAK1, JAK3, KDR, LAT, LCP2, PIK3R1, PLCG1, SRC, VAV1, ZAP70
• Immunogen: The immunogen for anti-GARS antibody: synthetic peptide directed towards the middle region of human GARS
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Guinea pig: 100%; Rabbit: 100%; Rat: 100%; Bovine: 92%; African clawed frog: 85%; Chicken: 85%; Zebrafish: 85%
• Peptide Sequence: Synthetic peptide located within the following region: IEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQN
• Blocking Peptide: For anti-GARS antibody is Catalog # AAP58758 (Previous Catalog # AAPP38525)
• Key Reference: Forster,C., (2008) Biochem. Biophys. Res. Commun. 368 (4), 1002-1006
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-GARS antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: GARS antibody tested with Human Mcf7 Cells (ARP58758_P050)

Aviva Systems Biology is the original manufacturer of this GARS antibody (ARP58758_P050)

Click here to view the GARS antibody Western Blot Protocol

Product Datasheet Link: GARS antibody (ARP58758_P050)

WB Suggested Anti-GARS Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: MCF7

Western Blot image:


Description of Target: GARS catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

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Aviva Systems Biology’s GARS antibody (ARP58758_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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