DFNA5 Antibody - N-terminal region (OAAB03945)

Catalog No: OAAB03945

Size:200UL

Western blot analysis of DFNA5 Antibody (N-term) in Hela cell lysates (35ug/lane). DFNA5 (arrow) was detected using the purified Pab.

DFNA5 is strongly supported by BioGPS gene expression data to be expressed in Hela

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with DFNA5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of MDA-MB468 cells using DFNA5 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: Western blot
• Additional Information: Cellular Location: Gasdermin-E, N-terminal: Cell membrane.
  Tissue Location: Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DFNA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-87 amino acids from the N-terminal region of human DFNA5.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Varies by lot. See vial for concentration.
• Application Info: WB: 1:1000
• Other Applications Image 1 Data: Flow cytometric analysis of MDA-MB468 cells using DFNA5 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: GSDME
• Gene Full Name: gasdermin E
• Alias Symbols: DFNA5;DFNA5, deafness associated tumor suppressor;gasdermin-E;ICERE-1;inversely correlated with estrogen receptor expression 1;nonsyndromic hearing impairment protein;non-syndromic hearing impairment protein 5.
• NCBI Gene Id: 1687
• Protein Name: NP_001120925.1, NP_001120926.1, NP_004394.1, O60443
• Description of Target: Hearing impairment is a heterogeneous condition with over 40 loci described. DFNA5 is expressed in fetal cochlea, however, its function is not known.
• Uniprot ID: https://www.uniprot.org/uniprot/O60443
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001120925.1,NP_001120926.1,NP_004394.1
• Molecular Weight: 55 kDa