- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for Cd63 Antibody (OASA04072) |
|---|
| Predicted Species Reactivity | Rat |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | AD1 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | Flow cytometry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
| Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8-653 myeloma cell line. Histology Positive Control Tissue: See reference 2 |
| :: | Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Preservative Stabilisers: 0.09% Sodium Azide (NaN3) |
| :: | Approx Protein Conc: IgG concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline |
| Reconstitution and Storage | -20°C |
| Immunogen | RBL-2H3 rat basophilic leukaemia cell line. |
| Purification | Protein A Purified |
| Predicted Homology Based on Immunogen Sequence | Rat |
| Concentration | 1 mg/ml |
| Specificity | OASA04072 specifically recognises rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, which is translocated to the cell surface upon activation of platelets, and therefore a potential indicator of platelet activation. Rat CD63 is expressed on mast cells, and studies using clone AD1, have shown that it is located near to FcER1, and that clone AD1 partially inhibits IgE-mediated histamine release, see Kitani, S. et al. for details. |
| Application Info | Flow Cytometry: 1/10 - 1/200 Use 10ul of the suggested working dilution to label 106 cells in 100ul. |
| Application Data | Application #1: Rat peripheral blood platelets stained with Mouse anti Rat CD63  Application #2: Rat peripheral blood platelets stained with Mouse anti Rat CD63 Alexa Fluor 488  Application #3: Rat peritoneal macrophages stained with Mouse anti Rat CD63:Alexa Fluor 647  |
| Reference | 1. Kitani, S. et al. (1991) A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491 J. Biol. Chem. 266: 1903-1909. 2. Boros, M. et al.. (1995) Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat. Shock. 3: 125-131. |
| Gene Symbol | Cd63 |
|---|---|
| Alias Symbols | MGC72893, Cd63 |
| NCBI Gene Id | 29186 |
| Protein Name | CD63 |
| Description of Target | MOUSE ANTI RAT CD63 |
| Uniprot ID | P28648 |
| Protein Accession # | NP_058821.1 |
| Protein Size (# AA) | 238 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Cd63 Antibody (OASA04072)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Rat".
-
How long will it take to receive "Cd63 Antibody (OASA04072)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
-
What buffer format is "Cd63 Antibody (OASA04072)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Cd63 Antibody (OASA04072)"?
This target may also be called "MGC72893, Cd63" in publications.
-
What is the shipping cost for "Cd63 Antibody (OASA04072)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Cd63 Antibody (OASA04072)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Cd63 Antibody (OASA04072)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Cd63 Antibody (OASA04072)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "CD63"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "CD63"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "CD63"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "CD63"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "CD63"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "CD63"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
