PDIA3 Antibody - middle region (OAAB01703)

Catalog No: OAAB01703

Size:400ul

Western blot analysis of PDIA3 Antibody (Center) in A2058, A375, Ramos cell lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.(2ug/ml)

Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (Center) (Cat. #OAAB01703). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2).

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of widr cells using PDIA3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Cow
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Cellular Location: Endoplasmic reticulum. Endoplasmic reticulum lumen (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
  Tissue Location: Detected in the flagellum and head region of spermatozoa (at protein level)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PDIA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the Central region of human PDIA3.
• Predicted Homology Based on Immunogen Sequence: Cow; Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of widr cells using PDIA3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: PDIA3
• Alias Symbols: P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, PI-PLC, HsT17083, HEL-S-269, HEL-S-93n
• NCBI Gene Id: 2923
• Protein Name: Protein disulfide-isomerase A3
• Uniprot ID: P30101
• Protein Accession #: NP_005304.3
• Molecular Weight: 56782 Da