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CHEK2 antibody - N-terminal region (ARP30290_P050)

  • Catalog#: ARP30290_P050
  • Domestic: within 1-2 days delivery International: 1-2 days

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100 ul
    $289.00
    In Stock

    Conjugation Options

    ARP30290_P050-FITC Conjugated

    ARP30290_P050-HRP Conjugated

    ARP30290_P050-Biotin Conjugated

    Free trial-size samples may be available for this item. Please go here for more information.
    Gene Symbol:
    CHEK2
    NCBI Gene Id:
    11200
    Official Gene Full Name:
    Checkpoint kinase 2
    Protein Name:
    Serine/threonine-protein kinase Chk2
    Swissprot Id:
    O96017-12
    Protein Accession #:
    NP_665861
    Nucleotide Accession #:
    NM_145862
    Alias Symbols:
    RP11-436C9.1, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
    Replacement Item:
    This antibody may replace item sc-136251 from Santa Cruz Biotechnology.
    Description of Target:
    In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
    Protein Size (# AA):
    514
    Molecular Weight:
    58kDa
    Host:
    Rabbit
    Clonality:
    Polyclonal
    Purification:
    Affinity Purified
    Application:
    IHC, WB
    Tissue Tool:
    Find tissues and cell lines supported by DNA array analysis to express CHEK2.
    RNA Seq:
    Find tissues and cell lines supported by RNA-seq analysis to express CHEK2.
    Immunogen:
    The immunogen is a synthetic peptide directed towards the N terminal region of human CHEK2
    Species Reactivity:
    Human
    Predicted Homology Based on Immunogen Sequence:
    Human: 100%
    Peptide Sequence:
    Synthetic peptide located within the following region: MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS
    Product Format:
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
    Reconstitution and Storage:
    For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
    Concentration:
    Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
    Protein Interactions:
    SRPK1; PPP2CA; PLK1; CHEK2; PRKDC; ATM; POLR2L; RCHY1; VHL; UBC; NR4A1; ELAVL1; CDC25A; ARHGAP1; PER3; CDC25C; CUL1; CUL4A; KPNA2; APP; STRAP; TP53; BRCA1; RB1; DAPK3; UBR5; PML; MDM2; BRCA2; KAT2B; LATS2; PSME3; TRIM28; RNF8; STAT1; PPP2R5E; PPP2R5D; PPP
    Blocking Peptide:
    For anti-CHEK2 (ARP30290_P050) antibody is Catalog # AAP30290 (Previous Catalog # AAPS08701)
    Datasheets / Downloads:
    Printable datasheet for anti-CHEK2 (ARP30290_P050) antibody
    Sample Type Confirmation:

    CHEK2 is supported by BioGPS gene expression data to be expressed in Jurkat

    Target Reference:
    Kobayashi,A., (2006) Histol. Histopathol. 21 (4), 373-382

    Product Protocols: CHEK2 antibody tested with Human Jurkat Cells (ARP30290_P050)

    Aviva Systems Biology is the original manufacturer of this CHEK2 antibody (ARP30290_P050)

    Click here to view the CHEK2 antibody Western Blot Protocol

    Product Datasheet Link: CHEK2 antibody (ARP30290_P050)

    WB Suggested Anti-CHEK2 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: Jurkat

    Western Blot image:


    Description of Target: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

    Questions pertaining to this data can be directed to techsupport@avivasysbio.com

    Aviva Systems Biology’s CHEK2 antibody (ARP30290_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

    To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

    All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

     
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