BRCA1 antibody - N-terminal region (ARP30434_P050)

Catalog#: ARP30434_P050

• Size: 50ug

Human ACHN

• Description of Target: The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.
• Gene Symbol: BRCA1
• Official Gene Full Name: Breast cancer 1, early onset
• Alias Symbols: BRCAI; BRCC1; IRIS; PSCP; RNF53; PNCA4; BROVCA1; PPP1R53
• Protein Accession#: NP_009229
• Nucleotide Accession#: NM_007298
• Swissprot Id: P38398
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 680
• Molecular Weight: 76kDa
• Application: WB
• Partner Proteins: ETS2,CREBBP,EP300,MAP3K3,MED1,BRCA1,SMAD2,SMAD3,SMAD4,UBB,ACACA,ATR,BRCA2,CCNA1,CCNB1,CCND1,CDK2,CDK4,CSNK2B,E2F1,E2F4,FANCA,HDAC1,HDAC2,JUN,JUNB,JUND,LMO4,MSH2,MSH6,MYC,NFYA,NMI,NUFIP1,POU2F1,RAD51,RAD51,RB1,RBBP4,RBBP7,RBBP8,SP1,TP53,TP53,ABL1,ACACA,AKT1,AR,ATF1,ATR,AURKA,BAP1,BARD1,BRAP,BRCA1,BRCA2,BRCC3,BRIP1,CASP3,CCNA1,CCNA2,CCNB1,CCND1,CDK1,CDK2,CDK4,CDK7,CHEK2,CLSPN,COBRA1,CREBBP,CSNK2A1,CSNK2B,CSTF1,CTBP1,DCLRE1C,DHX9,E2F1,E2F4,ELK1,EP300,ESR1,FAM175A,FANCA,FANCD2,FHL2,GFI1B,H2AFX,HDAC1,HDAC2,IFI16,JAK1,JAK2,JUN,JUNB,JUND,KPNA2,KPNA6,LMO4,MAP3K3,MDC1,MED1,MED21,MLH1,MNAT1,MYC,NBN,NFKB1,NFYA,NMI,NUFIP1,POLR2A,POLR2K,POU2F1,PPP1CA,PSAP,RAD51,RB1,RBBP4,RBBP7,RBBP8,RELA,RFC1,SMAD2,SMAD3,SMAD4,SMARCA2,SMARCA4,SMC1A,SP1,STAT1,STAT3,STAT5A,TOP2A,TP53,TP53BP1,TRRAP,TUBA4A,TUBB,TUBG1,UBB,UBE2D3,UBE2L3,USF2,VCP,XRCC1,YY1,ZNF350,ABL1,ACACA,AKT1,APLP2,AR,ARNT,ASM4,ATF1,ATM,ATR,AURKA,BACH1,BARD1,BLM,BRAP,BRCA1,BRCA2,BRCC3,BRD7,BRE,BRIP1,CCNA1,CCNA2,CCNB1,CCND1,CCNE1,CCR4,CDK1,CDK16,CDK2,CDK4,CDK5,CDK8,CDKN2D,CDS1,CHEK2,CLSPN,COBRA1,CREB1,CREBBP,CSNK2A1,CSNK2B,CSTF1,CSTF2,CTBP1,CTCFL,DEF1,DHH1,DHX9,DNAJA3,E2F4,ELK4,EP300,ESR1,ETS1,FANCA,FANCD2,FHL2,FLI1,FOXO3,GTF2E1,GTF2F1,GTF2H4,H2AFX,H2AFY,HDAC1,HDAC2,HIST2H2AC,HIST2H3C,HSPA8,Ifi204,JAK1,JAK2,JUN,JUNB,JUND,KAT2A,KIF1B,KPNA2,LMO4,MAP3K3,MAP4K4,MDC1,MED1,MED13,MED17,MED21,MED23,MED24,MLH1,MLP2,MRE11A,MRPL39,MSH2,MSH3,MSH6,MYC,NBN,NCOA2,NCOA3,NFYA,NMI,NPM1,NUFIP1,NUP120,NUP133,NUP153,NUP170,NUP188,NUP2,ORC2,ORC3,PEG3,PIAS1,PIAS4,POLR2A,POM34,POU2F1,PPP1CA,PPP1CB,PPP1R3A,PRKDC,RAD50,RAD51,RB1,RBBP4,RBBP7,RBBP8,RBL1,RBL2,RELA,RFC1,RPA1,RPL31,SMAD3,SMARCA2,SMARCA4,SMARCB1,SMARCC1,SMARCC2,SMARCD2,SP1,SPT4,SPT5,SRC,STAT1,STAT5A,SUB1,SUMO1,SUMO2,TERF1,TERF2,TOP2A,TOP2A,TP53,TP53BP1,TRRAP,TUBG1,UBA1,UBC,UBE2D1,UBE2I,UBE2J1,UBE2L3,UBE3A,UIMC1,USF2,VCP,WDR48,WNT2B,XIST,XRCC5,ZCCHC8,ZNF350
• Immunogen: The immunogen for anti-BRCA1 antibody: synthetic peptide directed towards the N terminal of human BRCA1
• Product Format: Lyophilized powder
• Predicted Homology Based on Immunogen Sequence: Human: 100%; Dog: 92%; Rat: 76%
• Peptide Sequence: Synthetic peptide located within the following region: DLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKL
• Blocking Peptide: For anti-BRCA1 antibody is Catalog # AAPP33639
• Key Reference: N/A
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-BRCA1 antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: BRCA1 antibody tested with Human Achn Cells (ARP30434_P050)

Aviva Systems Biology is the original manufacturer of this BRCA1 antibody (ARP30434_P050)

Click here to view the BRCA1 antibody Western Blot Protocol

Product Datasheet Link: BRCA1 antibody (ARP30434_P050)

WB Suggested Anti-BRCA1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: ACHN

Western Blot image:


Description of Target: The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.

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Aviva Systems Biology’s BRCA1 antibody (ARP30434_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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