NR2F2 Antibody - N-terminal region (ARP39465_T100)

Catalog No: ARP39465_T100

WB Suggested Antibody Titration: 2.5 ug/ml
Positive Control: MCF7NR2F2 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells

Host: Rabbit
Target Name: NR2F2
Sample Type: Human Fetal Kidney
Antibody Dilution: 1.0ug/ml

Human kidney

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Additional Information: IHC Information: MCF7 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NR2F2
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 79%
• Peptide Sequence: Synthetic peptide located within the following region: QDEVPGSQGSQASQAPPVPGPPPGAPHTPQTPGQGGPASTPAQTAAGGQG
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-NR2F2 (ARP39465_T100) antibody is Catalog # AAP39465 (Previous Catalog # AAPP21479)
• Sample Type Confirmation:

  NR2F2 is strongly supported by BioGPS gene expression data to be expressed in MCF7

• Reference: Sato,Y., (2003) J. Clin. Endocrinol. Metab. 88 (7), 3415-3420

Target Info

• Gene Symbol: NR2F2
• Gene Full Name: Nuclear receptor subfamily 2, group F, member 2
• Alias Symbols: ARP1, ARP-1, CHTD4, NF-E3, SRXX5, SVP40, COUPTF2, COUPTFB, TFCOUP2, COUPTFII
• NCBI Gene Id: 7026
• Protein Name: COUP transcription factor 2
• Description of Target: NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation.
• Uniprot ID: P24468
• Protein Accession #: NP_066285
• Nucleotide Accession #: NM_021005
• Protein Size (# AA): 414
• Molecular Weight: 45kDa
• Protein Interactions: NSD1; BCL11A; NR2F2; BIK; SETD7; FAM46A; HIPK3; UBC; TFAP4; Cebpb; SMARCAD1; NR2F6; POU5F1; NR3C1; PHB2; TRIP4; PIAS1; BCL11B; TRIM24; EP300; SQSTM1; HDAC1; NCOR2; MYOD1; LCK;