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Datasheets/Manuals | Printable datasheet for anti-GAPVD1 (ARP42451_P050) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | IHC, WB |
Additional Information | IHC Information: HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%. |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human GAPVD1 |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
Peptide Sequence | Synthetic peptide located within the following region: FKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPSQQ |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-GAPVD1 (ARP42451_P050) antibody is Catalog # AAP42451 (Previous Catalog # AAPP24794) |
Reference | Hunker,C.M., (2006) Biochem. Biophys. Res. Commun. 340 (3), 967-975 |
Gene Symbol | GAPVD1 |
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Gene Full Name | GTPase activating protein and VPS9 domains 1 |
Alias Symbols | RAP6, GAPEX5, GAPex-5 |
NCBI Gene Id | 26130 |
Protein Name | GTPase-activating protein and VPS9 domain-containing protein 1 |
Description of Target | GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. It acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. It is also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. It has GEF activity for Rab5 and GAP activity for Ras. |
Uniprot ID | Q14C86-6 |
Protein Accession # | NP_056450 |
Nucleotide Accession # | NM_015635 |
Protein Size (# AA) | 1487 |
Molecular Weight | 166kDa |
Protein Interactions | UBC; FOXK1; LRRC47; SF3A1; HNRNPR; IQGAP1; CCDC6; NR3C1; YWHAB; COPS2; SIRT7; ELAVL1; VAV2; RAD21; Csnk1e; CBL; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GAPVD1 Antibody - N-terminal region (ARP42451_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish".
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How long will it take to receive "GAPVD1 Antibody - N-terminal region (ARP42451_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "GAPVD1 Antibody - N-terminal region (ARP42451_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GAPVD1 Antibody - N-terminal region (ARP42451_P050)"?
This target may also be called "RAP6, GAPEX5, GAPex-5" in publications.
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What is the shipping cost for "GAPVD1 Antibody - N-terminal region (ARP42451_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GAPVD1 Antibody - N-terminal region (ARP42451_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GAPVD1 Antibody - N-terminal region (ARP42451_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "166kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GAPVD1 Antibody - N-terminal region (ARP42451_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GAPVD1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GAPVD1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GAPVD1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GAPVD1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GAPVD1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GAPVD1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.