DUT Antibody - C-terminal region (ARP46027_T100)

Catalog No: ARP46027_T100

WB Suggested Antibody Titration: 2.5ug/ml
Positive Control: JurkatDUT is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

Human Heart

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Additional Information: IHC Information: Jurkat cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 15%.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DUT
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Yeast: 93%; Zebrafish: 86%
• Peptide Sequence: Synthetic peptide located within the following region: NFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTGKN
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-DUT (ARP46027_T100) antibody is Catalog # AAP46027 (Previous Catalog # AAPP26870)
• Sample Type Confirmation:

  DUT is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Reference: Studebaker,A.W., (2005) Biochem. Biophys. Res. Commun. 327 (1), 306-310

Target Info

• Gene Symbol: DUT
• Gene Full Name: Deoxyuridine triphosphatase
• Alias Symbols: dUTPase
• NCBI Gene Id: 1854
• Protein Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
• Description of Target: DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
• Uniprot ID: P33316
• Protein Accession #: NP_001020419
• Nucleotide Accession #: NM_001025248
• Protein Size (# AA): 252
• Molecular Weight: 19kDa
• Protein Interactions: GDI2; NUDT18; PLEKHF2; C19orf25; MRPL14; LEMD3; UBL4A; RPL38; UBC; CUL3; ESRRG; ESRRA; ESR1; SPATA2; DUT; PPARD; PPARA; CDK1;