HDAC2 antibody - C-terminal region (ARP32618_P050)

Catalog#: ARP32618_P050

• Size: 50ug

Human Jurkat

Human Spermatophore

• Description of Target: Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.
• Gene Symbol: HDAC2
• Official Gene Full Name: Histone deacetylase 2
• Alias Symbols: HD2; RPD3; YAF1
• Protein Accession#: NP_001518
• Nucleotide Accession#: NM_001527
• Swissprot Id: Q92769
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 488
• Molecular Weight: 55kDa
• Application: WB, IHC
• Partner Proteins: SMURF1, TBP, ASH2L, DNMT1, HIST2H3C, HIST2H4A, KIFC1, MEN1, MLL, MLL2, RBBP5, SETD1A, SETD1B, WDR5, WDR77, WDR82, WDR82P1
• Immunogen: The immunogen for anti-HDAC2 antibody: synthetic peptide directed towards the C terminal of human HDAC2
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Bovine: 100%; Chicken: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Guinea pig: 92%; Horse: 92%; Rat: 92%; African clawed frog: 85%
• Peptide Sequence: Synthetic peptide located within the following region: EEFSDSEDEGEGGRRNVADHKKGAKKARIEEDKKETEDKKTDVKEEDKSK
• Blocking Peptide: For anti-HDAC2 antibody is Catalog # AAP32618 (Previous Catalog # AAPP03627)
• Key Reference: Choi, Y.B., et al., (2004) J.Biol.Chem.279(49),50930-50941
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-HDAC2 antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: HDAC2 antibody tested with Human Jurkat Cells (ARP32618_P050)

Aviva Systems Biology is the original manufacturer of this HDAC2 antibody (ARP32618_P050)

Click here to view the HDAC2 antibody Western Blot Protocol

Product Datasheet Link: HDAC2 antibody (ARP32618_P050)

WB Suggested Anti-HDAC2 Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat

Western Blot image:


Description of Target: Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s HDAC2 antibody (ARP32618_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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Product Protocols: HDAC2 antibody tested by IHC with human spermatophore (ARP32618)

Aviva Systems Biology is the original manufacturer of this HDAC2 antibody.

Click here to view the HDAC2 antibody Immunohistochemistry (IHC) protocol

Product Datasheet Link: HDAC2 antibody (ARP32618)

IHC Information:

Rabbit Anti-HDAC2 Antibody
Catalog Number: ARP32618
Paraffin Embedded Tissue: Human Spermatophore
Cellular Data: Epithelial cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

IHC Image: