ZIC2 Antibody - C-terminal region (ARP35821_P050)

Catalog No: ARP35821_P050

WB Suggested Anti-ZIC2 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate

Host: Rabbit
Target Name: ZIC2
Sample Tissue: Human HT1080 Whole Cell
Antibody Dilution: 1ug/ml

Human Skin

ZIC2 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with ARP35821_P050 with 1:200 dilution. Western blot was performed using ARP35821_P050 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: ZIC2 IP with ARP35821_P050 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Guinea Pig
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB, IP
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ZIC2
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Guinea Pig: 87%; Human: 100%; Mouse: 85%; Rat: 92%
• Peptide Sequence: Synthetic peptide located within the following region: EPQSSSNLSPAAAAAAAAAAAAAAAVSAVHRGGGSGSGGAGGGSGGGSGS
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-ZIC2 (ARP35821_P050) antibody is Catalog # AAP35821 (Previous Catalog # AAPP23577)
• Reference: Brown,L., (2005) Hum. Mol. Genet. 14 (3), 411-420
• Publications:

  Addiction of pancreatic cancer cells to zinc-finger transcription factor ZIC2. Oncotarget. 6, 28257-68 (2015). 26318045

  The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1. J Biol Chem. 293, 7982-7992 (2018). 29588366

  ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells. J. Clin. Invest. 125, 3795-808 (2015). 26426078

• Description:
  View product citations for ZIC2 Antibody - C-terminal region (ARP35821_P050) on CiteAb

Target Info

• Gene Symbol: ZIC2
• Gene Full Name: Zic family member 2
• Alias Symbols: HPE5
• NCBI Gene Id: 7546
• Protein Name: Zinc finger protein ZIC 2
• Description of Target: ZIC2 is a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects.This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects. This gene is closely linked to a gene encoding zinc finger protein of the cerebellum 5, a related family member on chromosome 13.
• Uniprot ID: O95409
• Protein Accession #: NP_009060
• Nucleotide Accession #: NM_007129
• Protein Size (# AA): 532
• Molecular Weight: 55kDa
• Protein Interactions: SUMO1; ELAVL1; GLI3; GLI2; GLI1; RNF180; UBC;