PCM1 Antibody - C-terminal region (OAAB04534)

Catalog No: OAAB04534

Size:400ul

Western blot analysis of PCM-1 antibody (C-eterm) in K562 cell lysates (35ug/lane). PCM1 (arrow) was detected using the purified Pab.

PCM1 is strongly supported by BioGPS gene expression data to be expressed in K562

Formalin-fixed and paraffin-embedded human lymph reacted with PCM-1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of K562 cells using PCM-1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function: Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia. {ECO:0000269|PubMed:12403812, ECO:0000269|PubMed:15659651, ECO:0000269|PubMed:16943179, ECO:0000269|PubMed:20551181, ECO:0000269|PubMed:24121310}.
  
  Cellular Location: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, cilium basal body. Note=Recruitement to the centrosome requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase Displaced from centriolar satellites and centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAP kinase signaling
  
  
  Tissue Location: Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid. {ECO:0000269|PubMed:10980597, ECO:0000269|PubMed:15184884, ECO:0000269|PubMed:16270321, ECO:0000269|PubMed:16424865}
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1816-1845 amino acids from the C-terminal region of human PCM-1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  PCM1 is strongly supported by BioGPS gene expression data to be expressed in K562

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of K562 cells using PCM-1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: PCM1
• Alias Symbols: PTC4, RET/PCM-1
• NCBI Gene Id: 5108
• Protein Name: Pericentriolar material 1 protein
• Uniprot ID: Q15154
• Protein Accession #: NP_006188.3
• Molecular Weight: 228533 Da