DDR2 Antibody (OAAB04761)

Catalog No: OAAB04761

Size:400ul

Western blot analysis of TYRO10 Antibody in Jurkat, 293 and Hela cell lysates (35ug/lane). TYRO10 (arrow) was detected using the purified Pab.

Western blot analysis of TYRO10 (arrow) using rabbit polyclonal TYRO10 Antibody either nontransfected (Lane 1) or lysates transiently transfected transfected with the DDR2 gene (Lane 2) (Origene Technologies).

DDR2 is strongly supported by BioGPS gene expression data to be expressed in Hela

Formalin-fixed and paraffin-embedded human normal lung with TYRO10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of jurkat cells using TYRO10 Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
• Additional Information: Function: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.
  Cellular Location: Cell membrane; Single- pass type I membrane protein
  Tissue Location: Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage (at protein level). Detected at high levels in heart and lung, and at low levels in brain, placenta, liver, skeletal muscle, pancreas, and kidney.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This TYRO10 antibody is generated from rabbits immunized with a his tag recombinant protein of human TYRO10.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  DDR2 is strongly supported by BioGPS gene expression data to be expressed in Hela

• Intended Use: TYRO10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of jurkat cells using TYRO10 Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: DDR2
• Alias Symbols: TKT, WRCN, MIG20a, NTRKR3, TYRO10
• NCBI Gene Id: 4921
• Protein Name: Discoidin domain-containing receptor 2
• Description of Target: Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane and cytoplasmic regions. There are several subclasses of RTKs and TYRO10 belongs to a novel subclass. The deduced amino acid sequence of TYRO10 has a unique extracellular region encompassing a factor VIII-like domain, not previously described for RTKs.
• Uniprot ID: Q16832
• Protein Accession #: NP_001014796.1
• Molecular Weight: 96736 Da