CCND1 antibody - middle region (AVARP03037_P050)

Catalog#: AVARP03037_P050

• Size: 50ug

Human 721_B

• Description of Target: CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Gene Symbol: CCND1
• Official Gene Full Name: Cyclin D1
• Alias Symbols: BCL1; D11S287E; PRAD1; U21B31
• Protein Accession#: NP_444284
• Nucleotide Accession#: NM_053056
• Swissprot Id: P24385
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 295
• Molecular Weight: 34kDa
• Application: WB
• Partner Proteins: AKAP8, AREG, ATF5, ATP5C1, CASP2, CDK11B, CDK14, CDK4, CDK6, CDKN1A, CDKN1B, CRABP2, DMTF1, DTNBP1, EFEMP2, EIF3K, MAF1, MAPK4, MCM10, NCOA2, NPDC1, PCNA, RARA, RBL2, RUNX1, VDR, AKAP8, ATP5C1, CASP2, CDK11B, CDK4, CDK6, CDKN1A, CDKN1B, CRABP2, DTNBP1, EFEMP2, EIF3K, MAF1, MCM10, NPDC1, PCNA, RAR
• Immunogen: The immunogen for anti-CCND1 antibody: synthetic peptide directed towards the middle region of human CCND1
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Guinea pig: 100%; Human: 100%; Mouse: 100%; Rat: 100%; African clawed frog: 90%; Bovine: 90%; Chicken: 90%; Dog: 90%; Horse: 90%; Zebrafish: 81%
• Peptide Sequence: Synthetic peptide located within the following region: ACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
• Blocking Peptide: For anti-CCND1 antibody is Catalog # AAP30439 (Previous Catalog # AAPP01023)
• Key Reference: Kibel,A.S., (2008) Prostate 68 (11), 1179-1186
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-CCND1 antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: CCND1 antibody tested with Human 721_B_Lymphoblast (AVARP03037_P050)

Aviva Systems Biology is the original manufacturer of this CCND1 antibody (AVARP03037_P050)

Click here to view the CCND1 antibody Western Blot Protocol

Product Datasheet Link: CCND1 antibody (AVARP03037_P050)

WB Suggested Anti-CCND1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B

Western Blot image:


Description of Target: CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

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Aviva Systems Biology’s CCND1 antibody (AVARP03037_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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