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CCND1 antibody - middle region (AVARP03037_P050)

  • Catalog#: AVARP03037_P050
  • Domestic: within 1-2 days delivery International: 1-2 days
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    - Trial Size Available. Trial size is not available for conjugation.
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100 ul
    $289.00
    In Stock

    Conjugation Options

    AVARP03037_P050-FITC Conjugated

    AVARP03037_P050-HRP Conjugated

    AVARP03037_P050-Biotin Conjugated

    Free trial-size samples may be available for this item. Please go here for more information.
    Gene Symbol:
    CCND1
    NCBI Gene Id:
    595
    Official Gene Full Name:
    Cyclin D1
    Protein Name:
    G1/S-specific cyclin-D1
    Swissprot Id:
    P24385
    Protein Accession #:
    NP_444284
    Nucleotide Accession #:
    NM_053056
    Alias Symbols:
    BCL1, D11S287E, PRAD1, U21B31
    Replacement Item:
    This antibody may replace item sc-166288 from Santa Cruz Biotechnology.
    Description of Target:
    CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
    Protein Size (# AA):
    295
    Molecular Weight:
    34kDa
    Host:
    Rabbit
    Clonality:
    Polyclonal
    Purification:
    Affinity Purified
    Application:
    WB
    Tissue Tool:
    Find tissues and cell lines supported by DNA array analysis to express CCND1.
    RNA Seq:
    Find tissues and cell lines supported by RNA-seq analysis to express CCND1.
    Immunogen:
    The immunogen is a synthetic peptide directed towards the middle region of human CCND1
    Species Reactivity:
    Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish
    Predicted Homology Based on Immunogen Sequence:
    Cow: 90%; Dog: 90%; Guinea Pig: 100%; Horse: 90%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 81%
    Complete computational species homology data:
    Anti-CCND1 (AVARP03037_P050)
    Peptide Sequence:
    Synthetic peptide located within the following region: ACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
    Product Format:
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
    Reconstitution and Storage:
    For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
    Concentration:
    Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
    Protein Interactions:
    CDKN1B; CDKN1A; CDK4; RELA; FBXO31; UBC; FBXO4; CAMK1; TP73; RB1; CDK2; HSPA8; POLR1B; MCM10; ORC4; LPL; BRINP1; CDK6; RABEP1; CARD11; CUL1; RBL2; UBTF; CCND1; ATF2; CDKN2A; FBXW8; BTRC; PPP1CC; PPP1CB; PSMA6; ITLN1; MCM7; MCM6; MCM3; MCM2; ELAVL1; PCNA;
    Blocking Peptide:
    For anti-CCND1 (AVARP03037_P050) antibody is Catalog # AAP30439 (Previous Catalog # AAPP01023)
    Datasheets / Downloads:
    Printable datasheet for anti-CCND1 (AVARP03037_P050) antibody
    Target Reference:
    Kibel,A.S., (2008) Prostate 68 (11), 1179-1186

    Product Protocols: CCND1 antibody tested with Human 721_B_Lymphoblast (AVARP03037_P050)

    Aviva Systems Biology is the original manufacturer of this CCND1 antibody (AVARP03037_P050)

    Click here to view the CCND1 antibody Western Blot Protocol

    Product Datasheet Link: CCND1 antibody (AVARP03037_P050)

    WB Suggested Anti-CCND1 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: 721_B

    Western Blot image:


    Description of Target: CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

    Questions pertaining to this data can be directed to techsupport@avivasysbio.com

    Aviva Systems Biology’s CCND1 antibody (AVARP03037_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

    To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

    All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

    Product Protocols: CCND1 antibody tested with Human SH-SY5Y Cells (AVARP03037_P050)

    Aviva Systems Biology is the original manufacturer of this CCND1 antibody (AVARP03037_P050)

    Product Datasheet Link: CCND1 antibody (AVARP03037_P050)

    WB Suggested Anti-CCND1 antibody Dilution: 1:1000 (stock 1mg/mL)
    Amount of lysate: 25ug
    Sample type: Human SH-SY5Y
    Blocking buffer: PBST-3% milk
    Primary antibody concentration: 1ug/mL
    Primary antibody incubation time: 4hrs at RT with shaking
    Secondary antibody: Goat anti-rabbit-Hrp
    Secondary antibody concentration: 0.1ug/mL
    Secondary antibody incubation time: 1hr at RT with shaking

    Western Blot image: 

    Description of Target: CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

    Questions pertaining to this data can be directed to techsupport@avivasysbio.com

    Aviva Systems Biology’s CCND1 antibody (AVARP03037_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

    To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

    All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

     
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