CCNB1 antibody - C-terminal region (ARP30161_P050)

Catalog#: ARP30161_P050

• Size: 50ug

Human Jurkat

Mouse Brain

Human NT2

• Description of Target: CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34(cdc2) to form the maturation-promoting factor (MPF).The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
• Gene Symbol: CCNB1
• Official Gene Full Name: Cyclin B1
• Alias Symbols: CCNB
• Protein Accession#: NP_114172
• Nucleotide Accession#: NM_031966
• Swissprot Id: P14635
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 433
• Molecular Weight: 48kDa
• Application: WB , IP
• Partner Proteins: CHUK, CREBBP, CTNNB1, ERCC3, ESR1, NCOA3, POLR2A, RELA, BRCA1, CDK4, CDK4, CDK6, CDKN1A, CDKN1A, CDKN1A, CDKN1B, KLF6, PIN1, RB1, TAF1, FOXO4, AKAP8, AR, BRCA1, CALM1, CCNDBP1, CDK4, CDK6, CDKN1A, CDKN1B, CTNNB1, CUL3, DMTF1, EP300, ESR1, GSK3B, HDAC3, HERC5, IFI27, INSM1, KAT2B, MCM10, MYBL2, NCO
• Immunogen: The immunogen for anti-CCNB1 antibody: synthetic peptide directed towards the C terminal of human CCNB1
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%; Bovine: 92%; Mouse: 92%; Pig: 92%; Rabbit: 92%; Rat: 92%; Dog: 84%
• Peptide Sequence: Synthetic peptide located within the following region: YTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNS
• Blocking Peptide: For anti-CCNB1 antibody is Catalog # AAP30161 (Previous Catalog # AAPS08606)
• Key Reference: Zhao,M., (2006) Exp Oncol 28 (1), 44-48
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-CCNB1 antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: CCNB1 antibody tested with Human Jurkat Cells (ARP30161_P050)

Aviva Systems Biology is the original manufacturer of this CCNB1 antibody (ARP30161_P050)

Click here to view the CCNB1 antibody Western Blot Protocol

Product Datasheet Link: CCNB1 antibody (ARP30161_P050)

WB Suggested Anti-CCNB1 Antibody Titration: 0.125ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat

Western Blot image:


Description of Target: CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34(cdc2) to form the maturation-promoting factor (MPF).The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s CCNB1 antibody (ARP30161_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Review: CCNB1 antibody-C-terminal region (ARP30161_P050) in Mouse brain homogenate using IP

Product Page for CCNB1 antibody - C-terminal region (ARP30161_P050)

Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Application: IP
Species+tissue/cell type: Mouse brain homogenate
How many ug’s of tissue/cell lysate run on the gel: 500 ug mouse brain homogenate
IP antibody: 6 ug
Primary antibody dilution: 1:500
Secondary antibody: Goat anti-rabbit Alexa-Fluor 594
Secondary antibody dilution: 1:5000

IP Protocol (Protein G coupled antibody):
Brain tissues were homogenized in a buffer containing 125 mM NaCl, 20mM Tris, pH7.4,
1mM MagCl2, 2% CHAP, 10% Glycerol, and protease inhibitor mirture.
Incubate on ice for 30 min.
Spin down 10 min at 14k rpm.
Add Protein G preclearance for 30 min on ice with rocking.
Spin down 5 min.
Add 6-12 of pre-coupled antibody-Protein G and rock 2 h on ice.
Spin down and wash 6 times.
Boil sample in sample buffer with beta-ME for 10 min.
Resolve on 4-20% Pierce Precise Protein gels.
Blot overnight with 2ug/ml antibodies.
Wash 2x7 min in TBS-tween.
Add secondary antibody (LiCor) for 1.5h.
Take images using LiCor.

Product Review: CCNB1 antibody-C-terminal region (ARP30161_P050) in human NT2 cell line using Western blot

Product Page for CCNB1 antibody - C-terminal region (ARP30161_P050)

Researcher:Dr. Yuzhi Chen, University of Arkansas for Medical Science
Application:Western blotting
Species+tissue/cell type: lane 1: 60ug human NT2 cell line
Primary antibody dilution: 1:500
Secondary antibody:IRDye 800 CW goat anti-rabbit from Li-COR Bioscience
Secondary antibody dilution:1:20,000