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Datasheets/Manuals | Printable datasheet for HRAS Antibody (OASA02910) |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. Phosphate buffered saline |
Clonality | Monoclonal |
Clone | H-Ras-03 |
Isotype | IgG1 |
Host | Mouse |
Application | Enzyme-linked immunosorbent assay|Western blot |
Additional Information | Preservative Stabilisers: 0.09% Sodium Azide (NaN3) |
:: | Approx Protein Conc: IgG concentration 1.0mg/ml Buffer Solutions: Phosphate buffered saline |
Reconstitution and Storage | -20°C |
Immunogen | Synthetic peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras. |
Purification | Purified IgG prepared by affinity chromatography on Protein A from ascites |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 1 mg/ml |
Specificity | Mouse anti Human H-Ras antibody, clone H-Ras-03 recognizes the human GTPase HRas also known as H-Ras, Transforming protein p21 or p21ras. H-Ras is a ubiquitously expressed lipid-anchored GTPase belonging to the Ras superfamily of small GTPases, which acts as a molecular signal transduction on/off switch on the inner surface of the plasma membrane and endomembranes (Milburn et al. 1990). H-Ras, N-Ras and K-Ras are highly related RAS genes which regulate pathways involved in cell differentiation, proliferation, adhesion, migration and apoptosis and are known proto-oncogenes. H-Ras is located within the peripheral plasma membrane and within endomembrane compartments including the ER and Golgi apparatus. In the ER H-Ras can be activated by the RasGRF family exchange factors RasGRF1 and RasGRF2, this process does not appear to happen withing the Golgi (Arozarena et al. 2004). Mutations in the H-Ras gene are responsible for the rare condition known as Costello syndrome characterized by facial, cardiovascular and musculoskeletal abnormalities and mental retardation (Gripp et al. 2006). Mouse anti Human H-Ras, clone H-Ras-03 has been used for the detection of H-Ras in the MCF10A human epitheliod breast cancer cell line by Western blotting (Vuoriluoto et al. 2011). |
Application Info | Western Blot: This product detects a band of approximately 24kDa in HeLa, K567 and RAJI whole cell lysates under reducing conditions. Suggested Dilution: 0.5-1.0 ug/ml |
Reference | 1. Zheng,Y. and Quilliam, L.A. (2003) Activation of the Ras superfamily of small GTPases. Workshop on exchange factors. EMBO reports. 4: 463-468. |
Gene Symbol | HRAS |
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Alias Symbols | CTLO, HAMSV, HRAS1, RASH1, p21ras, C-H-RAS, H-RASIDX, C-BAS/HAS, C-HA-RAS1 |
NCBI Gene Id | 3265 |
Protein Name | GTPase HRas |
Description of Target | MOUSE ANTI HUMAN H-Ras |
Uniprot ID | P01112 |
Protein Accession # | NP_001123914.1 |
Protein Size (# AA) | 189 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HRAS Antibody (OASA02910)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "HRAS Antibody (OASA02910)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "HRAS Antibody (OASA02910)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HRAS Antibody (OASA02910)"?
This target may also be called "CTLO, HAMSV, HRAS1, RASH1, p21ras, C-H-RAS, H-RASIDX, C-BAS/HAS, C-HA-RAS1" in publications.
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What is the shipping cost for "HRAS Antibody (OASA02910)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HRAS Antibody (OASA02910)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HRAS Antibody (OASA02910)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HRAS Antibody (OASA02910)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HRAS"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HRAS"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HRAS"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HRAS"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HRAS"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HRAS"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.