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GFI1B antibody - N-terminal region (ARP30093_P050)

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100 ul
$289.00
In Stock
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Gene Symbol:
GFI1B
NCBI Gene Id:
8328
Official Gene Full Name:
Growth factor independent 1B transcription repressor
Protein Name:
Zinc finger protein Gfi-1b
Swissprot Id:
Q5VTD9
Protein Accession #:
NP_004179
Nucleotide Accession #:
NM_004188
Description of Target:
GFI1B acts in the late stage of erythroid differentiation as a transcriptional repressor. GATA-1 and NF-Y both contribute to erythroid-specific transcriptional activation of the Gfi-1B promoter. This zinc finger protein mediates erythroid expansion and has a role in normal erythropoiesis.
Protein Size (# AA):
330
Molecular Weight:
38kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
IHC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GFI1B.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GFI1B.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human GFI1B
Species Reactivity:
Cow, Dog, Goat, Horse, Human, Mouse, Pig, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 83%; Dog: 100%; Goat: 83%; Horse: 100%; Human: 100%; Mouse: 83%; Pig: 93%; Rat: 82%
Complete computational species homology data:
Anti-GFI1B (ARP30093_P050)
Peptide Sequence:
Synthetic peptide located within the following region: MPRSFLVKSKMAHTYHQPRVQEDEPLWPPALTPVPRDQAPSNSPVLSTLF
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
ZNRD1; EFEMP2; NSMF; IFFO1; RCHY1; TENC1; HECW1; MID2; CCDC85B; COPS5; RUNDC3A; PDLIM5; FBLN5; MATR3; PDE4DIP; BRE; PICK1; HGS; DNAJA3; KALRN; BECN1; LTBP4; VSNL1; TRIP6; TRAF1; TMF1; TAF1; ATXN2; TRIM27; PSMA3; NOTCH1; NELL2; NELL1; LAMB1; JAG2; HSPG2; H
Blocking Peptide:
For anti-GFI1B (ARP30093_P050) antibody is Catalog # AAP30093 (Previous Catalog # AAPH00269)
Datasheets / Downloads:
Printable datasheet for anti-GFI1B (ARP30093_P050) antibody
Target Reference:
Huang,D.Y., et al., (2004) Nucleic Acids Res. 32 (13), 3935-3946

Product Protocols: GFI1B antibody tested with Human Jurkat Cells (ARP30093_P050)

Aviva Systems Biology is the original manufacturer of this GFI1B antibody (ARP30093_P050)

Click here to view the GFI1B antibody Western Blot Protocol

Product Datasheet Link: GFI1B antibody (ARP30093_P050)

WB Suggested Anti-GFI1B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat

Western Blot image:


Description of Target: GFI1B acts in the late stage of erythroid differentiation as a transcriptional repressor. GATA-1 and NF-Y both contribute to erythroid-specific transcriptional activation of the Gfi-1B promoter. This zinc finger protein mediates erythroid expansion and has a role in normal erythropoiesis.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s GFI1B antibody (ARP30093_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocols: GFI1B antibody tested by IHC with human kidney (ARP30093)

Aviva Systems Biology is the original manufacturer of this GFI1B antibody.

Click here to view the GFI1B antibody Immunohistochemistry (IHC) protocol

Product Datasheet Link: GFI1B antibody (ARP30093)

IHC Information:

Rabbit Anti-GFI1B Antibody
Catalog Number: ARP30093
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule and renal corpuscle
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

IHC Image:

Product Protocols: GFI1B antibody tested by IHC with human heart (ARP30093)

Aviva Systems Biology is the original manufacturer of this GFI1B antibody.

Click here to view the GFI1B antibody Immunohistochemistry (IHC) protocol

Product Datasheet Link: GFI1B antibody (ARP30093)

IHC Information:

Rabbit Anti-GFI1B Antibody
Catalog Number: ARP30093
Paraffin Embedded Tissue: Human Heart
Cellular Data: Myocardial cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

IHC Image:

 
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