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Datasheets/Manuals | Printable datasheet for anti-MECP2 (ARP38539_P050) antibody |
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Tested Species Reactivity | Human, Mouse |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human MECP2 |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 75% |
Peptide Sequence | Synthetic peptide located within the following region: EPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPV |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-MECP2 (ARP38539_P050) antibody is Catalog # AAP38539 (Previous Catalog # AAPP20730) |
Reference | Loat,C., (er) Genes Brain Behav. (2008) In press |
Publications | Anti-MeCP2 ARP38539_P050 has recently been referenced in the following publications: Zhao, J. et al. Epigenetic silence of ankyrin-repeat-containing, SH3-domain-containing, and proline-rich-region- containing protein 1 (ASPP1) and ASPP2 genes promotes tumor growth in hepatitis B virus-positive hepatocellular carcinoma. Hepatology 51, 142–53 (2010). ChIP, Human 20034025 |
Gene Symbol | MECP2 |
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Gene Full Name | Methyl CpG binding protein 2 (Rett syndrome) |
Alias Symbols | RS, RTS, RTT, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13 |
NCBI Gene Id | 4204 |
Protein Name | Methyl-CpG-binding protein 2 |
Description of Target | Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. |
Uniprot ID | P51608 |
Protein Accession # | NP_004983 |
Nucleotide Accession # | NM_004992 |
Protein Size (# AA) | 486 |
Molecular Weight | 52kDa |
Protein Interactions | UBC; HECW2; Hipk2; SOX18; WHSC1; HPDL; XPC; CAT; HIST2H3C; HIST1H4F; HIST2H2BE; HIST2H2AC; CBX3; CBX1; SMC3; YBX1; SIN3A; CBX5; ELAVL1; SUMO2; PPARG; YY1; TERF2; SMARCA2; SOX2; SP1; SP3; LOC407840; CDKL5; PRPF40A; SMARCB1; NCOR1; SKI; HMGB1; SPI1; LBR; RB |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MECP2 Antibody - middle region (ARP38539_P050)"?
The tested species reactivity for this item is "Human, Mouse". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish".
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How long will it take to receive "MECP2 Antibody - middle region (ARP38539_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "MECP2 Antibody - middle region (ARP38539_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MECP2 Antibody - middle region (ARP38539_P050)"?
This target may also be called "RS, RTS, RTT, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13" in publications.
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What is the shipping cost for "MECP2 Antibody - middle region (ARP38539_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MECP2 Antibody - middle region (ARP38539_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MECP2 Antibody - middle region (ARP38539_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "52kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MECP2 Antibody - middle region (ARP38539_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MECP2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MECP2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MECP2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MECP2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MECP2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MECP2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.