MCM4 Antibody - N-terminal region (ARP36662_T100)

Catalog No: ARP36662_T100

WB Suggested Anti-MCM4 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysateMCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MCM4
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 92%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%
• Peptide Sequence: Synthetic peptide located within the following region: SRVEGTPRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSL
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-MCM4 (ARP36662_T100) antibody is Catalog # AAP36662 (Previous Catalog # AAPP07919)
• Sample Type Confirmation:

  MCM4 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Reference: Ishimi,Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650

Target Info

• Gene Symbol: MCM4
• Gene Full Name: Minichromosome maintenance complex component 4
• Alias Symbols: NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21
• NCBI Gene Id: 4173
• Protein Name: DNA replication licensing factor MCM4
• Description of Target: The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
• Uniprot ID: P33991
• Protein Accession #: NP_877423
• Nucleotide Accession #: NM_182746
• Protein Size (# AA): 863
• Molecular Weight: 95kDa
• Protein Interactions: HUWE1; UBC; PCNA; SUMO2; FKBP5; MCMBP; MDM2; POLK; CLUH; NCKAP1; CAP1; CAP2; EIF3B; MCM2; KPNA3; PRMT1; FKBP4; MCM7; MCM6; FBXW4; FBXO46; ITGA4; CDC5L; gag-pol; ORC6; CCNA1; VCAM1; DBF4; CDC7; LIG4; MCM5; MCM3; DCTN2; SF3A1; UBD; NEDD8; TONSL; SIRT7; ASF1