MBD2 Antibody - middle region (ARP40169_T100)

Catalog No: ARP40169_T100

WB Suggested Anti-MBD2 Antibody Titration: 0.625ug/ml
ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate

Lanes:
Lane 1: 15ug WT mouse ES lysate
Lane 2: 15ug MBD2 KO mouse ES lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-rabbit-HRP
Secondary Antibody Dilution:
1:2500
Gene Name:
MBD2 a
Submitted by:
Austin J. Cooney, Baylor College of Medicine

Rabbit Anti-MBD2 Antibody
Catalog Number: ARP40169_T100
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MBD2
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: DCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNT
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-MBD2 (ARP40169_T100) antibody is Catalog # AAP40169 (Previous Catalog # AAPP20912)
• Reference: Le (2006) Mol. Cell. Biol. 26 (3), 843-851

Target Info

• Gene Symbol: MBD2
• Gene Full Name: Methyl-CpG binding domain protein 2
• Alias Symbols: DMTase, NY-CO-41
• NCBI Gene Id: 8932
• Protein Name: Methyl-CpG-binding domain protein 2
• Description of Target: MBD2 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD2 can also repress transcription from methylated gene promoters. MBD2 may function as a mediator of the biological consequences of the methylation signal.It is also reported that the MBD2 functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
• Uniprot ID: Q9UBB5-3
• Protein Accession #: NP_056647
• Nucleotide Accession #: NM_015832
• Protein Size (# AA): 302
• Molecular Weight: 32kDa
• Protein Interactions: UBC; SOX2; HDAC2; HDAC1; MTA3; LAMTOR3; TFAP4; KDM5B; CDK2AP1; ESR1; KDM1A; MTA2; MBD2; RBBP7; RBBP4; CHD3; tat; MBD3L2; MBD3; csul; GATAD2A; TACC3; PRMT5; WDR77; PRMT1; PTK2B; TRIM27; GATAD2B; MBD3L1; GPN1; SIN3A; HINFP; DHX9; CREBBP; DNMT1; tax; NEWENTR