CYP3A5 Antibody - middle region (OAAB04851)

Catalog No: OAAB04851

Size:400ul

Western blot analysis of CYP3A5 Antibody (Center) in 293 cell lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP3A5 Antibody (Center) (Cat. #OAAB04851), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

CYP3A5 Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  
  Cellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CYP3A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-218 amino acids from the Central region of human CYP3A5.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: CYP3A5 Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: CYP3A5
• Alias Symbols: CP35, PCN3, CYPIIIA5, P450PCN3
• NCBI Gene Id: 1577
• Protein Name: Cytochrome P450 3A5
• Uniprot ID: P20815
• Protein Accession #: NP_000768.1
• Molecular Weight: 57109 Da