- Description of Target:
- Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
- Gene Symbol:
- DFFA
- Official Gene Full Name:
- DNA fragmentation factor, 45kDa, alpha polypeptide
- Alias Symbols:
- DFF-45; DFF1; Ion ChannelAD; ICAD
- Tissue Tool:
- Find tissues and cell lines supported to express DFFA.
- Protein Accession# :
- NP_004392
- Nucleotide Accession#:
- NM_004401
- Swissprot Id:
- O00273
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Protein Size (# AA):
- 331
- Molecular Weight:
- 37kDa
- Application:
- WB
- Subunit:
- alpha
- Partner Proteins:
- BAK1, BCL6, APEX1, ATF3, ATM, ATM, ATM, ATM, ATR, AURKA, BAK1, BARD1, BAX, BAX, BAX, BCL2L1, BLM, BLM, BLM, BNIP3L, BRCA1, BRCA1, CARM1, CDC25C, CDKN1A, CDKN1A, CDKN1A, CDKN1A, CDKN1A, CR2, CREBBP, CUL9, DDX5, DHCR24, EP300, EP300, EP300, ERCC3, ESR1, FOXO3, GPS2, GSK3B, HCVgp1, HCVgp1, HCVgp1, HCVg
- Immunogen:
- The immunogen for anti-DFFA antibody: synthetic peptide directed towards the N terminal of human DFFA
- Product Format:
- Lyophilized powder
- Purification:
- Protein A purified
- Complete computational species homology data:
- DFFA antibody - N-terminal region (ARP30359_T100)
- Predicted Homology Based on Immunogen Sequence:
- Human: 100%; Bovine: 91%; Guinea pig: 91%; Rat: 91%; Dog: 83%; Mouse: 83%
- Datasheets / Downloads:
- Printable datasheet for
anti-DFFA antibody
- ARP30359_T100 - Peptide Sequence:
- Synthetic peptide located within the following region: MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDIL
- Blocking Peptide:
- For anti-DFFA antibody is Catalog # AAP30359 (Previous Catalog # AAPS08806)
- Key Reference:
- Yan,B., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (5), 1504-1509
- Reconstitution and Storage:
- Add 100 μl of distilled water. Final anti-DFFA antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Product Protocols: DFFA antibody tested with Human Jurkat Cells (ARP30359_T100)
Aviva Systems Biology is the original manufacturer of this DFFA antibody (ARP30359_T100)
Click here to view the DFFA antibody Western Blot Protocol
Product Datasheet Link: DFFA antibody (ARP30359_T100)
WB Suggested Anti-DFFA Antibody Titration: 5.0ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat
Western Blot image: 
Description of Target: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Questions pertaining to this data can be directed to techsupport@avivasysbio.com
Aviva Systems Biology’s DFFA antibody (ARP30359_T100) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.
To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.
All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com
- Protocol:
- Tips Information:
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