ALKBH8 Antibody - C-terminal region (OAAB03896)

Catalog No: OAAB03896

Size:400ul

Western blot analysis of ALKBH8 Antibody (C-term) in HL60 cell lysates (35ug/lane). ALKBH8 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinica

Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
  
  Cellular Location: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic
  Tissue Location: Widely expressed, with highest expression in spleen, followed by pancreas and lung
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ALKBH8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from the C-terminal region of human ALKBH8.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: ALKBH8
• Alias Symbols: ABH8, TRM9, MRT71, TRMT9, TRMT9A
• NCBI Gene Id: 91801
• Protein Name: Alkylated DNA repair protein alkB homolog 8
• Uniprot ID: Q96BT7
• Protein Accession #: NP_620130.2
• Molecular Weight: 75208 Da