SRF Antibody - N-terminal region (P100866_T100)

Catalog No: P100866_T100

WB Suggested Anti-SRF Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysateSRF is supported by BioGPS gene expression data to be expressed in Jurkat

Chromatin Immunoprecipitation (ChIP) Using SRF antibody - N-terminal region (P100866_T100) and HCT116 Cells

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: CHIP, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SRF
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: ATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIM
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-SRF (P100866_T100) antibody is Catalog # AAP31232 (Previous Catalog # AAPP01977)
• Sample Type Confirmation:

  SRF is supported by BioGPS gene expression data to be expressed in Jurkat

• Reference: Doi,H., et al., (2005) Arterioscler. Thromb. Vasc. Biol. 25 (11), 2328-2334
• Description:
  View product citations for SRF Antibody - N-terminal region (P100866_T100)on CiteAb

Target Info

• Gene Symbol: SRF
• Gene Full Name: Serum response factor (c-fos serum response element-binding transcription factor)
• Alias Symbols: MCM1
• NCBI Gene Id: 6722
• Protein Name: Serum response factor
• Description of Target: SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).
• Uniprot ID: P11831
• Protein Accession #: NP_003122
• Nucleotide Accession #: NM_003131
• Protein Size (# AA): 508
• Molecular Weight: 52kDa
• Protein Interactions: Camk2d; ZIC3; ELK1; SRFBP1; SRF; NFKB1; PRKDC; CIRBP; ALDH3A1; SUMO1; UBE2I; SHBG; TRIM63; KDM6A; MYOCD; MKL1; CNTN2; CSNK2A1; SMARCA4; PELP1; KAT5; HDAC4; EPC1; SMAD7; HDAC3; MKL2; ATF6; BARX2; MAPKAPK2; FHL3; GATA4; CAMK2A; HOPX; PYCARD; NKX2-3; MINPP1;