HNRPA0 Antibody - middle region (ARP40686_T100)

Catalog No: ARP40686_T100

WB Suggested Anti-HNRPA0 Antibody Titration: 0.625ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate

Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate
Primary Antibody Dilution:
1:4000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
HNRPA0
Submitted by:
Anonymous

Rabbit Anti-HNRPA0 Antibody
Catalog Number: ARP40686
Paraffin Embedded Tissue: Human Heart
Cellular Data: Myocardial cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Rabbit Anti-HNRPA0 Antibody
Catalog Number: ARP40686
Paraffin Embedded Tissue: Human Liver
Cellular Data: Hepatocytes
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Rabbit Anti-HNRNPA0 Antibody
Catalog Number: ARP40686
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Sample Type:
MCF7 cells
Primary Antibody Dilution:
1:200
Secondary Antibody:
Anti-rabbit-FITC
Secondary Antibody Dilution:
1:500
Color/Signal Descriptions:
DAPI: Blue hn-RNPA0: Green
Gene Name:
HNRPA0
Submitted by:
Anonymous

Amount and Sample Type:
Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPA0 ppt. k562 sample
IP Antibody:
HNRPA0
Amount of IP Antibody:

Primary Antibody:
HNRPA0
Primary Antibody Dilution:
1:4000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
HNRPA0
Submitted by:
Anonymous

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Cow, Dog, Pig
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IF, IP, WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HNRPA0
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Human: 100%; Pig: 93%
• Peptide Sequence: Synthetic peptide located within the following region: KAAVVKFHPIQGHRVEVKKAVPKEDIYSGGGGGGSRSSRGGRGGRGRGGG
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-HNRNPA0 (ARP40686_T100) antibody is Catalog # AAP40686 (Previous Catalog # AAPY00938)
• Reference: Lai,F., (2001) Genomics 71 (2), 235-245

Target Info

• Gene Symbol: HNRNPA0
• Gene Full Name: Heterogeneous nuclear ribonucleoprotein A0
• Alias Symbols: HNRPA0
• NCBI Gene Id: 10949
• Protein Name: Heterogeneous nuclear ribonucleoprotein A0
• Description of Target: HNRPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.
• Uniprot ID: Q13151
• Protein Accession #: NP_006796
• Nucleotide Accession #: NM_006805
• Protein Size (# AA): 305
• Molecular Weight: 34kDa
• Protein Interactions: UBC; SUMO2; SUMO3; STAU1; RPA3; RPA2; RPA1; EED; RNF2; DYNC1LI1; PSMD13; PSMD12; PSMD8; MAP2; DYNC1I2; DHX9; ABCF1; RPS27; TARDBP; CSF2; NPM1; LMNA; AICDA; MAPKAPK2; GADD45A; U2AF2; VCAM1; SRSF1; NKX3-1; MTNR1A; ITGA4; IL7R; HNRNPK; HNRNPH3; HNRNPH2; HNRN