ZYX Antibody - middle region (ARP34388_P050)

Catalog No: ARP34388_P050

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WB Suggested Anti-ZYX Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Stomach

WB Suggested Anti-ZYX antibody Titration: 1 ug/mL
Sample Type: Human heart

Rabbit Anti-ZYX Antibody
Catalog Number: ARP34388_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult liver
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.

Rabbit Anti-ZYX Antibody
Catalog Number: ARP34388_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic (cytoskeleton)
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.

Rabbit Anti-ZYX Antibody
Catalog Number: ARP34388_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic in mitochondria
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZYX
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Rabbit: 79%; Rat: 86%
• Peptide Sequence: Synthetic peptide located within the following region: QPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPE
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-ZYX (ARP34388_P050) antibody is Catalog # AAP34388 (Previous Catalog # AAPY00230)
• Reference: Martynova,N.Y., (2008) Dev. Dyn. 237 (3), 736-749

Target Info

• Gene Symbol: ZYX
• Gene Full Name: Zyxin
• Alias Symbols: ESP-2, HED-2
• NCBI Gene Id: 7791
• Protein Name: Zyxin
• Description of Target: TRIM50 is an E3 ubiquitin-protein ligase.Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
• Uniprot ID: Q15942
• Protein Accession #: NP_003452
• Nucleotide Accession #: NM_003461
• Protein Size (# AA): 572
• Molecular Weight: 61kDa
• Protein Interactions: RTN4IP1; YPEL3; IMP3; PHF21A; PDLIM5; LIN7A; SUMO1; NEDD8; FAM129B; CORO1B; CAND1; PUF60; PAPOLA; NUDC; SEC23B; SEC24C; HIST1H4A; UGP2; TARS; PXN; GART; GARS; APEH; FN1; HIPK3; VCAM1; UBC; UBL7; UFC1; UBXN7; ZC3H7B; TTLL12; FERMT2; TOMM34; AHCYL1; SSSCA1;