- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for anti-ZP3 (ARP35855_T100) antibody |
---|
Tested Species Reactivity | Human |
---|---|
Predicted Species Reactivity | Human, Rat, Cow, Horse, Rabbit |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human ZP3 |
Purification | Protein A purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Horse: 100%; Human: 100%; Rabbit: 100%; Rat: 90% |
Peptide Sequence | Synthetic peptide located within the following region: NKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGATDLPGQEW |
Concentration | 1.0 mg/ml |
Blocking Peptide | For anti-ZP3 (ARP35855_T100) antibody is Catalog # AAP35855 (Previous Catalog # AAPP07111) |
Reference | Chamberlin,M.E., et al., (1990) Proc. Natl. Acad. Sci. U.S.A. 87 (16), 6014-6018 |
Publications | Morphometric characteristics of preantral and antral follicles and expression of factors involved in folliculogenesis in ovaries of adult baboons (Papio anubis). 26945754 Törmälä, R.-M. et al. Zona pellucida components are present in human fetal ovary before follicle formation. Mol. Cell. Endocrinol. 289, 10-5 (2008). 18502569 |
Gene Symbol | ZP3 |
---|---|
Gene Full Name | Zona pellucida glycoprotein 3 (sperm receptor) |
Alias Symbols | ZPC, ZP3A, ZP3B, Zp-3, OOMD3 |
NCBI Gene Id | 7784 |
Protein Name | Zona pellucida sperm-binding protein 3 |
Description of Target | The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene |
Uniprot ID | P21754-2 |
Protein Size (# AA) | 424 |
Molecular Weight | 47kDa |
Protein Interactions | HECW2; OR2L13; SPACA3; MICALL1; MRPS16; PCDH17; UBAP2L; UBC; ENGASE; ACR; FURIN; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "ZP3 Antibody - C-terminal region (ARP35855_T100)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Rat, Cow, Horse, Rabbit".
-
How long will it take to receive "ZP3 Antibody - C-terminal region (ARP35855_T100)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
-
What buffer format is "ZP3 Antibody - C-terminal region (ARP35855_T100)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ZP3 Antibody - C-terminal region (ARP35855_T100)"?
This target may also be called "ZPC, ZP3A, ZP3B, Zp-3, OOMD3" in publications.
-
What is the shipping cost for "ZP3 Antibody - C-terminal region (ARP35855_T100)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "ZP3 Antibody - C-terminal region (ARP35855_T100)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "ZP3 Antibody - C-terminal region (ARP35855_T100)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "47kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ZP3 Antibody - C-terminal region (ARP35855_T100)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "ZP3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "ZP3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "ZP3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "ZP3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "ZP3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "ZP3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.