VCL Antibody - C-terminal region (ARP61099_P050)

Catalog No: ARP61099_P050

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WB Suggested Anti-VCL Antibody
Titration: 1.0 ug/ml
Positive Control: HepG2 Whole Cell

Rabbit Anti-VCL Antibody
Catalog Number: ARP61099_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Plasma membrane in intercalated disk
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human VCL
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: PRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAA
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-VCL (ARP61099_P050) antibody is Catalog # AAP61099 (Previous Catalog # AAPP47264)

Target Info

• Gene Symbol: VCL
• Gene Full Name: Vinculin
• Alias Symbols: MV, MVCL, CMD1W, CMH15, HEL114
• NCBI Gene Id: 7414
• Protein Name: Vinculin
• Description of Target: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
• Uniprot ID: P18206
• Protein Accession #: NP_003364
• Nucleotide Accession #: NM_003373
• Protein Size (# AA): 1066
• Molecular Weight: 117kDa
• Protein Interactions: VCL; UBC; TEKT1; SUMO2; ASB7; CTPS1; GINS3; UBFD1; ISOC1; DCPS; ERP44; PDCD10; FERMT2; YWHAQ; TUBB3; PDIA4; STK24; YWHAZ; YWHAH; YWHAG; YWHAE; XPO1; SNRPF; RAP1GDS1; PPP5C; PPP1R2; PFAS; PDHA1; MSN; HSPA9; HSPA5; GSS; SFN; GNAI3; G6PD; EIF5; ECHS1; TBCB;