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Datasheets/Manuals | Printable datasheet for anti-RFXAP (ARP33226_P050) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human, Guinea Pig |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human RFXAP |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Guinea Pig: 100%; Human: 100% |
Peptide Sequence | Synthetic peptide located within the following region: SDQALNCGGTASTGSAGNVKLEESADNILSIVKQRTGSFGDRPARPTLLE |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-RFXAP (ARP33226_P050) antibody is Catalog # AAP33226 (Previous Catalog # AAPP04263) |
Reference | Long,A.B., (2006) Mol. Immunol. 43 (5), 395-409 |
Gene Symbol | RFXAP |
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Gene Full Name | Regulatory factor X-associated protein |
Alias Symbols | - |
NCBI Gene Id | 5994 |
Protein Name | Regulatory factor X-associated protein |
Description of Target | RFXAP is part of the RFX complex that binds to the X-box of MHC II promoters.Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Uniprot ID | O00287 |
Protein Accession # | NP_000529 |
Nucleotide Accession # | NM_000538 |
Protein Size (# AA) | 272 |
Molecular Weight | 28kDa |
Protein Interactions | EPS8; NHP2L1; NCL; MLLT1; HDAC4; SMARCA4; RFXANK; RFX5; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "RFXAP Antibody - middle region (ARP33226_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Guinea Pig".
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How long will it take to receive "RFXAP Antibody - middle region (ARP33226_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "RFXAP Antibody - middle region (ARP33226_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "RFXAP Antibody - middle region (ARP33226_P050)"?
This target may also be called "-" in publications.
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What is the shipping cost for "RFXAP Antibody - middle region (ARP33226_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "RFXAP Antibody - middle region (ARP33226_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "RFXAP Antibody - middle region (ARP33226_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "28kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "RFXAP Antibody - middle region (ARP33226_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RFXAP"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RFXAP"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RFXAP"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RFXAP"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RFXAP"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RFXAP"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.