POLR2I Antibody - N-terminal region (ARP35790_P050)

Catalog No: ARP35790_P050

WB Suggested Anti-POLR2I Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysatePOLR2I is supported by BioGPS gene expression data to be expressed in HepG2

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLR2I
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 83%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: ACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQ
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-POLR2I (ARP35790_P050) antibody is Catalog # AAP35790 (Previous Catalog # AAPP07042)
• Sample Type Confirmation:

  POLR2I is supported by BioGPS gene expression data to be expressed in HepG2

• Subunit: RPB9
• Reference: Grimwood,J., (2004) Nature 428 (6982), 529-535

Target Info

• Gene Symbol: POLR2I
• Gene Full Name: Polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• Alias Symbols: RPB9, hRPB14.5
• NCBI Gene Id: 5438
• Protein Name: DNA-directed RNA polymerase II subunit RPB9
• Description of Target: POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: P36954
• Protein Accession #: NP_006224
• Nucleotide Accession #: NM_006233
• Protein Size (# AA): 125
• Molecular Weight: 14kDa
• Protein Interactions: ESR1; TCEB3; SRC; PSMB9; UBC; MED19; MED26; RECQL5; TBCD; BRCA1; CPSF6; POLR2E; POLR2D; POLR2C; POLR2B; POLR2A; DCUN1D1; CUL5; MYC; tat; WDR48; POLR2H; MED10; MED29;