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NFKBIB antibody - middle region (ARP34381_P050)

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100 ul
$289.00
In Stock

Conjugation Options

ARP34381_P050-FITC Conjugated

ARP34381_P050-HRP Conjugated

ARP34381_P050-Biotin Conjugated

Gene Symbol:
NFKBIB
NCBI Gene Id:
4793
Official Gene Full Name:
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Protein Name:
NF-kappa-B inhibitor beta
Swissprot Id:
Q15653
Protein Accession #:
NP_002494
Nucleotide Accession #:
NM_002503
Alias Symbols:
IKBB, TRIP9
Replacement Item:
This antibody may replace item sc-113183 from Santa Cruz Biotechnology.
Description of Target:
Inhibition of NFkappa-B activity by the hepatitis C virus core protein might be related to its physical interaction with and interrupted nuclear localization of IKKbeta. Increased nuclear factor-kappaB (NF-kB) activity in the amnion during labor is associated with an increase in the expression of NF-kBp65 and of the NF-kB binding proteins IkBa, IkBb-1 and IkBb-2. IkappaBbeta may be a novel target for transcription factors of the HMG-box SRY/Sox family and imply a potential role for NF-kappaB/IkappaBbeta in spermatogenesis. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-766 BM449613.1 1-766 767-1189 BU634404.1 18-440 c 1190-1198 AI684894.1 1-9 c
Protein Size (# AA):
356
Molecular Weight:
38kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express NFKBIB.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express NFKBIB.
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human NFKBIB
Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 91%; Rat: 100%
Complete computational species homology data:
Anti-NFKBIB (ARP34381_P050)
Peptide Sequence:
Synthetic peptide located within the following region: PILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRS
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
VPS52; UBC; BAG3; TSPAN1; GIT2; RELA; REL; CSNK2A1; CDC25A; BAK1; APP; ZBTB7A; DNAJA3; IKBKB; CHUK; BRAP; IKBKG; TRE17; NFKBIA; NCOR2; PPARG; ESR2; POLR1D; POLR1E; POLR1A; POLR1C; POLR2H; NKIRAS2; POLR1B; NKIRAS1; LRPPRC; RASAL2; MTIF2; BTRC; CUL1; SKP1;
Blocking Peptide:
For anti-NFKBIB (ARP34381_P050) antibody is Catalog # AAP34381 (Previous Catalog # AAPY00223)
Datasheets / Downloads:
Printable datasheet for anti-NFKBIB (ARP34381_P050) antibody
Sample Type Confirmation:

NFKBIB is strongly supported by BioGPS gene expression data to be expressed in 721_B

Target Reference:
Kerppola,T.K. (er) Ann. Rheum. Dis. (2008) In press

Product Protocols: NFKBIB antibody tested with Human 721_B_Lymphoblast (ARP34381_P050)

Aviva Systems Biology is the original manufacturer of this NFKBIB antibody (ARP34381_P050)

Click here to view the NFKBIB antibody Western Blot Protocol

Product Datasheet Link: NFKBIB antibody (ARP34381_P050)

WB Suggested Anti-NFKBIB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B

Western Blot image:


Description of Target: Inhibition of NFkappa-B activity by the hepatitis C virus core protein might be related to its physical interaction with and interrupted nuclear localization of IKKbeta. Increased nuclear factor-kappaB (NF-kB) activity in the amnion during labor is associated with an increase in the expression of NF-kBp65 and of the NF-kB binding proteins IkBa, IkBb-1 and IkBb-2. IkappaBbeta may be a novel target for transcription factors of the HMG-box SRY/Sox family and imply a potential role for NF-kappaB/IkappaBbeta in spermatogenesis. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-766 BM449613.1 1-766 767-1189 BU634404.1 18-440 c 1190-1198 AI684894.1 1-9 c

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s NFKBIB antibody (ARP34381_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: NFKBIB Antibody (ARP34381_P050) in Human Testis Tissue using Immunohistochemistry

Aviva Systems Biology is the original manufacturer of this NFKBIB antibody.
Product Datasheet Link: NFKBIB antibody ARP34381_P050

Rabbit Anti-NFKBIB Antibody
Catalog Number: ARP34381_P050
Formalin Fixed Paraffin Embedded Tissue: Human Testis Tissue
Observed Staining: Nucleus, Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Left to right:
DAPI, NFKBIB Ab, Merge

Control Image:
Control Antibody: Normal Rabbit IgG
Control Antibody Concentration: 1:100

Left to right:
DAPI, Rabbit IgG, Merge

Protocol:
1. Normal adult human testis tissue was formalin fixed, embedded in paraffin wax, sectioned at 6 micron thickness and put on histological slides.
2. After deparaffinization and rehydration, the low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.
3. The blocking buffer was 5% normal goat serum.
4. Primary antibodies was diluted in antibody dilution buffer (1% Normal Donkey Serum) to the final testing dilution (1:100, 1:600, 1:1200).
5. The appropriate anti-rabbit fluorescent-conjugated (Rhodamine:red or FITC:green) secondary antibody was applied and nuclei will be counterstained with DAPI (blue).
6. A Negative control utilized a nonspecific rabbit IgG staining the same normal adult human testis tissue.

 
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